University of Strathclyde, SUPA - Scottish Universities Physics Alliance, Department of Physics, Centre for Molecular Nanometrology, Photophysics Group, John Anderson Building, 107 Rottenrow, Glasgow, G4 0NG United Kingdom.
J Biomed Opt. 2010 Mar-Apr;15(2):020504. doi: 10.1117/1.3366646.
Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging.
双光子荧光（TPL）来自金纳米棒在生物成像中具有相当大的潜力。我们使用荧光寿命成像显微镜（FLIM）研究金纳米棒在 Madin-Darby 犬肾（MDCK）细胞中的成像。FLIM 提供的图像具有比强度成像更好的对比度和灵敏度。发现金纳米棒的特征荧光寿命小于 100 ps，可用于在寿命成像中区分金纳米棒与其他荧光标记物和内源性荧光团。
