Barnoy Eran A, Fixler Dror, Popovtzer Rachela, Nayhoz Tsviya, Ray Krishanu
Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan 5290002, Israel.
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Nano Res. 2015 Dec 1;8(12):3912-3921. doi: 10.1007/s12274-015-0891-y.
In this study we developed a highly sensitive dual modal imaging system designed for gold nanoparticles (GNPs) conjugated to various fluorophores in solid phantoms. The system consists of fluorescence lifetime imaging microscopy (FLIM) for surface imaging, diffusion reflection (DR) for deep tissue imaging (up to 1cm), and metal enhanced fluorescence (MEF). We detected quenching in fluorescent intensity (FI) for the conjugation of gold nanospheres (GNS) as well as gold nanorods (GNRs) to Fluorescein, which has an excitation peak at a wavelength shorter than the surface plasmon resonance (SPR) of both types of GNPs, and enhanced FI in conjugation to Rhodamine B and Sulforhodamine B, both with excitation peaks in the GNPs' SPR. The enhanced FI was detected in solution as well as in solid phantoms from FLIM measurements. DR measurements detected GNR presence within the solid phantoms by recording dropped rates of light scattering using wavelengths corresponding to the GNRs' absorption. With the inclusion of MEF, this promising dual modal imaging technique enables efficient and sensitive molecular and functional imaging.
在本研究中，我们开发了一种高灵敏度双模态成像系统，该系统专为与固体模型中各种荧光团共轭的金纳米颗粒（GNP）设计。该系统由用于表面成像的荧光寿命成像显微镜（FLIM）、用于深度组织成像（深度达1厘米）的漫反射（DR）以及金属增强荧光（MEF）组成。我们检测到，当金纳米球（GNS）以及金纳米棒（GNR）与荧光素共轭时荧光强度（FI）淬灭，荧光素的激发峰波长比这两种类型的GNP的表面等离子体共振（SPR）波长短；而当与罗丹明B和磺基罗丹明B共轭时FI增强，这两种物质的激发峰均在GNP的SPR范围内。通过FLIM测量在溶液以及固体模型中均检测到了增强的FI。DR测量通过记录使用与GNR吸收对应的波长时的光散射下降率来检测固体模型中GNR的存在。通过加入MEF，这种有前景的双模态成像技术能够实现高效且灵敏的分子和功能成像。
