Abrahamsson S O, Lundborg G, Lohmander L S
Department of Hand Surgery, Malmö General Hospital, Sweden.
J Orthop Res. 1991 Jul;9(4):495-502. doi: 10.1002/jor.1100090405.
Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (Emax) of up to three times the control value was observed with rhIGF-I at 250 ng/ml. Maximal stimulation was observed at 5,000 ng/ml with insulin, and FCS at 15%. rhIGF-I was more potent than insulin in stimulating protein synthesis and cell proliferation. The Emax of stimulation of proteoglycan and collagen synthesis by rhIGF-I were two times that of FCS, and the Emax of cell proliferation by FCS was twice that of rhIGF-I. Growth factors thus have the ability to stimulate matrix synthesis and cell proliferation in rabbit flexor tendon. This provides a rationale for further studies on the role of growth factors in flexor tendon healing in humans.
屈肌腱具有内在的修复能力，能够代谢基质成分并进行增殖。为了确定可能影响这些能力的因素，我们在兔深屈肌腱的短期外植体培养中研究了重组人胰岛素样生长因子（rhIGF-I）、胰岛素和胎牛血清（FCS）对蛋白聚糖、胶原蛋白和非胶原蛋白合成以及细胞增殖的影响。rhIGF-I在10至250 ng/ml的剂量下、胰岛素在250至5000 ng/ml的剂量下以及FCS在2%至15%的浓度下，均呈剂量依赖性地刺激基质合成和细胞增殖。在250 ng/ml的rhIGF-I作用下，观察到估计的最大刺激（Emax）高达对照值的三倍。胰岛素在5000 ng/ml时以及FCS在15%时观察到最大刺激。rhIGF-I在刺激蛋白合成和细胞增殖方面比胰岛素更有效。rhIGF-I刺激蛋白聚糖和胶原蛋白合成的Emax是FCS的两倍，而FCS刺激细胞增殖的Emax是rhIGF-I的两倍。因此，生长因子具有刺激兔屈肌腱基质合成和细胞增殖的能力。这为进一步研究生长因子在人类屈肌腱愈合中的作用提供了理论依据。
