Abrahamsson S O, Lohmander S
Department of Hand Surgery, Malmö General Hospital, Sweden.
J Orthop Res. 1996 May;14(3):370-6. doi: 10.1002/jor.1100140305.
Tendon healing and integration of tendon grafts may be site or donor specific. To determine if differences exist in sensitivity to growth factors that have the potential to influence tendon repair, we compared the effects of recombinant human insulin-like growth factor-I on various types of tendon segments. The dose response effects on proteoglycan, collagen, noncollagen protein, and DNA synthesis were investigated in short-term explant cultures of intrasynovial intermediate and proximal segments of deep flexor tendons extrasynovial segments of deep flexor tendons, and Achilles tendons of rabbits. The four different types of tendon segments cultured in media without recombinant human insulin-like growth factor-I synthesized similar amounts of each of the matrix components. Intrasynovial proximal segments synthesized 15 times less DNA than other tendon segments. Recombinant human insulin-like growth factor-I stimulated matrix and DNA synthesis of all tendon segments in a dose-dependent manner in intervals from 10 to 1,000 ng/ml. The potency (LogED50) of the stimulation did not differ between the segments. The estimated maximal stimulation (E(max)) of proteoglycan synthesis by recombinant human insulin-like growth factor-I was higher, and of collagen and noncollagen protein synthesis was lower, in intrasynovial proximal segments as compared with that of the other types of segments. In contrast, the estimated maximal stimulation of DNA synthesis by recombinant human insulin-like growth factor-I was 6-fold higher than controls in all types of tendons. These findings demonstrate differences in mitotic capacity between anatomical regions of tendons during culture without recombinant human insulin-like growth factor-I and in matrix synthesis after stimulation with it.
肌腱愈合以及肌腱移植物的整合可能因部位或供体而异。为了确定对可能影响肌腱修复的生长因子的敏感性是否存在差异，我们比较了重组人胰岛素样生长因子-I对各种类型肌腱节段的影响。在兔深屈肌腱滑膜内中间段和近端段、深屈肌腱滑膜外段以及跟腱的短期外植体培养中，研究了其对蛋白聚糖、胶原蛋白、非胶原蛋白和DNA合成的剂量反应效应。在没有重组人胰岛素样生长因子-I的培养基中培养的四种不同类型的肌腱节段合成的每种基质成分的量相似。滑膜内近端段合成的DNA比其他肌腱节段少15倍。重组人胰岛素样生长因子-I以剂量依赖的方式刺激所有肌腱节段的基质和DNA合成，浓度范围为10至1000 ng/ml。各节段之间刺激的效价（LogED50）没有差异。与其他类型的节段相比，重组人胰岛素样生长因子-I对滑膜内近端段蛋白聚糖合成的估计最大刺激（E(max)）较高，而对胶原蛋白和非胶原蛋白合成的估计最大刺激较低。相反，重组人胰岛素样生长因子-I对所有类型肌腱DNA合成的估计最大刺激比对照高6倍。这些发现表明在没有重组人胰岛素样生长因子-I培养期间，肌腱不同解剖区域的有丝分裂能力存在差异，在用其刺激后基质合成也存在差异。
