State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Proteome Sci. 2011 Mar 8;9:11. doi: 10.1186/1477-5956-9-11.
Avian infectious bronchitis (IB) is one of the most serious diseases of economic importance in chickens; it is caused by the avian infectious coronavirus (IBV). Information remains limited about the comparative protein expression profiles of chicken embryonic tissues in response to IBV infection in ovo. In this study, we analyzed the changes of protein expression in trachea and kidney tissues from chicken embryos, following IBV infection in ovo, using two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS).
17 differentially expressed proteins from tracheal tissues and 19 differentially expressed proteins from kidney tissues were identified. These proteins mostly related to the cytoskeleton, binding of calcium ions, the stress response, anti-oxidative, and macromolecular metabolism. Some of these altered proteins were confirmed further at the mRNA level using real-time RT-PCR. Moreover, western blotting analysis further confirmed the changes of annexin A5 and HSPB1 during IBV infection.
To the best of our knowledge, we have performed the first analysis of the proteomic changes in chicken embryonic trachea and kidney tissues during IBV infection in ovo. The data obtained should facilitate a better understanding of the pathogenesis of IBV infection.
禽传染性支气管炎（IB）是鸡群中最重要的经济疾病之一，由禽传染性冠状病毒（IBV）引起。关于 IBV 感染鸡胚时鸡胚组织中比较蛋白质表达谱的信息仍然有限。在这项研究中，我们使用二维凝胶电泳（2-DE）结合基质辅助激光解吸/电离飞行时间串联质谱（MALDI-TOF-TOF MS）分析了 IBV 感染鸡胚后气管和肾脏组织中蛋白质表达的变化。
从气管组织中鉴定出 17 种差异表达蛋白，从肾脏组织中鉴定出 19 种差异表达蛋白。这些蛋白主要与细胞骨架、钙离子结合、应激反应、抗氧化和大分子代谢有关。其中一些改变的蛋白在 mRNA 水平上通过实时 RT-PCR 进一步得到证实。此外，Western blot 分析进一步证实了 annexin A5 和 HSPB1 在 IBV 感染过程中的变化。
据我们所知，我们首次分析了 IBV 感染鸡胚时鸡胚气管和肾脏组织的蛋白质组变化。获得的数据应有助于更好地了解 IBV 感染的发病机制。
