证据表明 Lfc 和 Tctex-1 参与了轴突的形成。
Evidence for the involvement of Lfc and Tctex-1 in axon formation.
机构信息
Instituto de Investigación Médica Mercedes y Martín Ferreyra-Consejo Nacional de Investigaciones Científicas y Técnicas, 5016 Cordoba, Argentina.
出版信息
J Neurosci. 2010 May 12;30(19):6793-800. doi: 10.1523/JNEUROSCI.5420-09.2010.
Abstract
RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation.
摘要
RhoA 和 Rac 在神经元极化过程中发挥关键作用，且作用相反。我们现在发现，Lfc（鸟嘌呤核苷酸交换因子（GEF））定位于发育中神经元的高尔基体和生长锥，通过 Rho 信号通路负调控神经突发芽和轴突形成。Tctex-1（一种参与轴突生长的动力蛋白轻链，通过调节肌动蛋白动力学和 Rac 活性）与 Lfc 共定位并相互作用，从而抑制其 GEF 活性，降低 Rho-GTP 水平，并在神经突形成过程中发挥功能拮抗作用。
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