National Herbarium of the Netherlands, Leiden University branch, P.O. Box 9514, 2300 RA Leiden, The Netherlands;
Persoonia. 2008 Jun;20:1-7. doi: 10.3767/003158508X283692. Epub 2008 Feb 1.
A phylogenetic study of the species in Boletus sect. Boletus was undertaken using the molecular markers ITS1-5.8S-ITS2 and GAPDH. Four well-supported lineages, one comprising Boletus edulis s.l., the others referring to B. aereus, B. reticulatus and B. pinophilus have been distinguished. The ML and MP trees of ITS showed remarkably low resolution within the B. edulis clade, and confirmed earlier published results, despite the use of samples from a wider geographical area and different hosts. The results of GAPDH demonstrate clearly that this low resolution must be ascribed to a low genetic variability with the B. edulis clade, and make clear that morphological and ecological characters have been overestimated within this species complex. Boletus edulis is therefore defined as a variable species with a wide morphological, ecological and geographic range, and includes several specific and subspecific taxa described in the literature (e.g. B. betulicola, B. persoonii, B. quercicola and B. venturii). Three other European species (B. aereus, B. pinophilus and B. reticulatus) are well delimited species based on morphology and our genetic data.
采用 ITS1-5.8S-ITS2 和 GAPDH 分子标记对 Boletus 属 Boletus 节的物种进行了系统发育研究。已经区分出四个支持良好的谱系，其中一个包括 Boletus edulis s.l.，其他三个分别指 B. aereus、B. reticulatus 和 B. pinophilus。ITS 的 ML 和 MP 树在 Boletus edulis 分支内分辨率非常低，尽管使用了更广泛地理区域和不同宿主的样本，但证实了早期发表的结果。GAPDH 的结果清楚地表明，这种低分辨率必须归因于 Boletus edulis 分支的遗传变异性低，并且清楚地表明形态和生态特征在该种复合体中被高估了。因此，Boletus edulis 被定义为一个具有广泛形态、生态和地理范围的可变物种，并包括文献中描述的几个特定和亚种分类群（例如 B. betulicola、B. persoonii、B. quercicola 和 B. venturii）。另外三个欧洲物种（B. aereus、B. pinophilus 和 B. reticulatus）根据形态和我们的遗传数据是很好界定的物种。
