Department of Anatomy & Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, United States.
Semin Cancer Biol. 2010 Aug;20(4):261-8. doi: 10.1016/j.semcancer.2010.05.003. Epub 2010 May 15.
Significant emphasis has recently been placed on the characterization of the human cancer genome. This effort has been assisted by the development of new DNA sequencing technologies that allow the genomes of individual tumors to be analyzed in much greater detail. However, the genetic complexity of human cancer has complicated the identification of driver mutations among the more abundant passenger mutations found in tumors. Recently, the Sleeping Beauty (SB) transposon system has been engineered to model cancer in mice. SB-induced tumors are produced by transposon insertional mutagenesis, thus the tagged mutations facilitate the identification of novel cancer genes. This review provides a brief summary of the SB system and its use in modeling cancer in mice.
近年来,人们非常重视对人类癌症基因组的特征描述。新的 DNA 测序技术的发展为此提供了帮助,这些技术可以更详细地分析单个肿瘤的基因组。然而,人类癌症的遗传复杂性使得在肿瘤中更常见的乘客突变中识别驱动突变变得复杂。最近,睡眠美人(SB)转座子系统被设计用于在小鼠中模拟癌症。SB 诱导的肿瘤是通过转座子插入诱变产生的,因此标记的突变有助于鉴定新的癌症基因。本文简要总结了 SB 系统及其在小鼠癌症模型中的应用。