Gantait Arunava, Pandit Subrata, Nema Neelesh K, Mukjerjee Pulok K
Jadavpur University, School of Natural Product Studies, Kolkata-700 032, India.
J AOAC Int. 2010 Mar-Apr;93(2):492-5.
Glycyrrhiza glabra Linn (Family-Fabaceae) is active as an anti-allergic, anti-inflammatory, spasmolytic, mild laxative, antistress, antidepressive, antiulcer, liver protective, estrogenic, emmenagogue, and antidiabetic substance, and is widely used in the Indian system of medicine. The major bioactive constituent is glycyrrhizin. A simple HPTLC method has been developed to control the quality of raw as well as finished glycyrrhiza using glycyrrhizin as the bioactive marker. The solvent system was optimized to chloroform-methanol-water (65 + 36 + 7.5, v/v/v). Extract and standard were dissolved in 70% methanol and applied on a precoated TLC plate. After development, the plate was scanned at 254 nm to create a chromatogram, then the quantity of glycyrrhizin was determined in the extract. The method was validated in terms of specificity, linearity, precision, LOD, and LOQ. Linearity range was found to be 0.96-4.80 microg per spot. The linearity relationship was described by the equation: Y = 612.706 + 1.091X (with r = 0.99904 and SD = 2.52%), where Y is the area under curve and X is the amount of glycyrrhizin (ng). The amount of glycyrrhizin found in the extract was 9.1%. Thus, the method provides a rapid and cost-effective quality measure for Glycyrrhiza glabra hydroalcoholic extract.
光果甘草（豆科）具有抗过敏、抗炎、解痉、轻度缓泻、抗应激、抗抑郁、抗溃疡、肝脏保护、雌激素样、通经和抗糖尿病等活性，在印度医学体系中广泛应用。主要生物活性成分是甘草甜素。已开发出一种简单的高效薄层色谱法，以甘草甜素作为生物活性标志物来控制生甘草及甘草成品的质量。优化后的溶剂系统为氯仿 - 甲醇 - 水（65 + 36 + 7.5，v/v/v）。将提取物和标准品溶于70%甲醇中，点样于预涂薄层板上。展开后，在254nm处扫描薄层板以生成色谱图，然后测定提取物中甘草甜素的含量。该方法在特异性、线性、精密度、检测限和定量限方面进行了验证。线性范围为每点0.96 - 4.80μg。线性关系由方程Y = 612.706 + 1.091X描述（r = 0.99904，SD = 2.52%），其中Y为曲线下面积，X为甘草甜素的量（ng）。提取物中甘草甜素的含量为9.1%。因此，该方法为光果甘草水醇提取物提供了一种快速且经济高效的质量检测方法。
