Institute of Oncology and Radiology of Serbia, Serbia.
J Med Food. 2010 Aug;13(4):851-62. doi: 10.1089/jmf.2009.0193.
Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines--human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453--and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.
红葡萄酒和白葡萄酒多酚已被报道具有显著的健康益处。在这项研究中，评估了红葡萄酒和白葡萄酒多酚提取物和白藜芦醇对不同癌细胞系（人宫颈腺癌 HeLa、人乳腺腺癌 MDA-MB-361 和人乳腺癌 MDA-MB-453）和正常人外周血单个核细胞（PBMCs）的细胞毒性活性。通过相应葡萄酒的分馏真空蒸馏获得的葡萄酒多酚提取物的定性和定量组成，使用分光光度法和高效液相色谱法与二极管阵列检测和液相色谱法与电喷雾电离飞行时间质谱分析来确定。结果表明，与 MDA-MB-361 细胞和未刺激和刺激的 PBMCs 相比，葡萄酒多酚提取物和白藜芦醇对 HeLa 和 MDA-MB-453 细胞表现出更高的细胞毒性活性。此外，白葡萄酒多酚提取物对癌细胞系的增殖抑制作用明显高于红葡萄酒提取物。用橙黄素和溴化乙锭混合物染色预处理的白葡萄酒提取物处理的 HeLa 细胞中出现浓缩或碎裂的核，表明细胞凋亡的形态学迹象。此外，还观察到处于凋亡晚期或继发性坏死的 HeLa 细胞。我们的研究结果表明，红葡萄酒和白葡萄酒中的多酚提取物可能具有抗癌潜力。
