1st Department of Pediatrics, Semmelweis University, Budapest, Hungary.
Working Group Integrative Kidney Physiology, Institute of Physiology, Charité-University Medicine Berlin, Berlin, Germany.
Methods Mol Biol. 2021;2216:45-55. doi: 10.1007/978-1-0716-0978-1_3.
Renal diseases remain devastating illnesses with unacceptably high rates of mortality and morbidity worldwide. Animal models are essential tools to better understand the pathomechanism of kidney-related illnesses and to develop new, successful therapeutic strategies. Magnetic resonance imaging (MRI) has been actively explored in the last decades for assessing renal function, perfusion, tissue oxygenation as well as the degree of fibrosis and inflammation. This chapter aims to provide an overview of the preparation and monitoring of small animals before, during, and after surgical interventions or MR imaging. Standardization of experimental settings such as body temperature or hydration of animals and minimizing pain and distress are essential for diminishing nonexperimental variables as well as for conducting ethical research.This publication is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers.
肾脏疾病仍然是一种毁灭性的疾病，全球范围内的死亡率和发病率都高得令人无法接受。动物模型是更好地了解与肾脏相关疾病的发病机制和开发新的、成功的治疗策略的重要工具。在过去的几十年中，磁共振成像（MRI）一直被积极探索用于评估肾功能、灌注、组织氧合以及纤维化和炎症程度。本章旨在概述在手术干预或 MRI 之前、期间和之后对小动物的准备和监测。动物的体温或水合作用的标准化以及最小化疼痛和不适是减少非实验变量以及进行伦理研究的关键。本出版物基于 COST 行动 PARENCHIMA 的工作，该行动是一个由社区驱动的网络，由欧盟的欧洲科学技术合作组织（COST）计划资助，旨在提高肾脏 MRI 生物标志物的重现性和标准化。
