Department of Pharmacy, Anhui Medical University, Hefei, Hong Kong, China.
J Pharm Pharmacol. 2010 Feb;62(2):223-30. doi: 10.1211/jpp.62.02.0010.
It was found that total flavonoids from Litsea coreana Levl. (TFLC), which is a traditional Chinese medicine, had a preventive effect against hepatic steatosis in our previous study. This study was designed to evaluate whether TFLC could improve liver fibrosis in rats.
The liver fibrosis model rats were treated with composite factors of high-fat emulsion (10 ml/kg) via gavage accompanied by a subcutaneous injection of low-dose CCl(4). Thirty rats were given composite factors plus TFLC (100, 200, 400 mg/kg), respectively, for 8 weeks.
The results showed that TFLC (200 and 400 mg/kg) treatment significantly reduced the elevation of liver index (liver weight/body weight) and spleen index (spleen weight/body weight), alanine transaminase, aspartate aminotransferase, hyaluronic acid, laminin, procollagen III N-terminal peptide, procollagenase IV and hydroxyproline. In addition, TFLC treatment improved the morphologic changes of hepatic fibrosis, suppressed expression of alpha-smooth muscle actin, collagen I, transforming growth factor (TGF)-beta1 and TGFbeta receptor (TGFbetaR)1, and increased peroxisome proliferator-activated receptor-gamma expression in the liver of hepatic fibrosis rats.
In conclusion, TFLC is able to ameliorate liver injury and protect rats from liver fibrosis. This process may be related to inhibiting the expression of transforming growth factor-beta1 and increasing the expression of peroxisome proliferator-activated receptor-gamma.
我们之前的研究发现，来自中国传统草药香叶树（Litsea coreana Levl.）的总黄酮（TFLC）对肝脂肪变性具有预防作用。本研究旨在评估 TFLC 是否可改善大鼠肝纤维化。
采用高脂乳剂（10 ml/kg）灌胃联合小剂量 CCl(4)皮下注射复合因素建立肝纤维化模型大鼠，给予复合因素加 TFLC（100、200、400 mg/kg）治疗 8 周。
结果表明，TFLC（200 和 400 mg/kg）治疗可显著降低肝指数（肝重/体重）和脾指数（脾重/体重）、丙氨酸转氨酶、天冬氨酸转氨酶、透明质酸、层粘连蛋白、III 型前胶原 N 端肽、脯氨酸酶 IV 和羟脯氨酸的升高。此外，TFLC 治疗可改善肝纤维化的形态变化，抑制α-平滑肌肌动蛋白、胶原 I、转化生长因子（TGF）-β1 和 TGFβ 受体（TGFbetaR）1 的表达，并增加肝纤维化大鼠肝脏过氧化物酶体增殖物激活受体-γ的表达。
总之，TFLC 可改善肝损伤并保护大鼠免受肝纤维化的影响。这一过程可能与抑制转化生长因子-β1 的表达和增加过氧化物酶体增殖物激活受体-γ的表达有关。
