School of Pharmacy, Anhui Medical University, Key Laboratory for Bioactivity of Natural Medicine of Anhui Province, Hefei, China.
Am J Chin Med. 2012;40(3):599-610. doi: 10.1142/S0192415X12500450.
Alcoholic fatty liver (AFL) is a reversible condition, but it can potentiate the development of alcoholic hepatitis and even cirrhosis by increasing oxidant generation, which is one of the key pathogenic factors and could result in alcoholic liver disease (ALD). Total flavonoids from Litsea coreana (TFLC), an active component extracted from Litsea coreana leve, have been shown to have therapeutic effects on hyperlipidemia. The present study was to evaluate the protective effects of TFLC on alcoholic fatty liver (AFL) in rats, and investigate the potential mechanism. An AFL model in rats was established by intaking different doses of alcohol (concentration from 5% to 40%) over 12 weeks. Serum levels of TG, TC, LDL-C, HDL-C, TNF-α, insulin, and glucose were measured, histopathologic changes were determined, and expression of adipose differentiation-related protein (ADRP) in the liver were evaluated by Western blotting and immunohistochemistry, respectively. The results showed that treatment with TFLC resulted in decreased serum levels of TG, TC, LDL-C, TNF-α, glucose and insulin, as well as improved liver index. Morphological evaluation revealed rats in model group developed a severe steatosis, but the severities of liver steatosis were effectively ameliorated in TFLC (200 and 400 mg/kg) treated groups. Expression of hepatic ADRP were increased in model group, and suppressed in TFLC treated groups. These results suggest that TFLC had a protective effect on AFL rats; the mechanism may be involved in regulation serum lipid profiles via down-regulation of hepatic expression of ADRP in AFL rats.
酒精性脂肪肝（AFL）是一种可逆转的疾病，但它可以通过增加氧化应激的产生而促进酒精性肝炎甚至肝硬化的发展，这是关键的致病因素之一，并可能导致酒精性肝病（ALD）。从山鸡椒中提取的总黄酮（TFLC）是山鸡椒叶片中的一种活性成分，已被证明对高脂血症具有治疗作用。本研究旨在评估 TFLC 对大鼠酒精性脂肪肝（AFL）的保护作用，并探讨其潜在机制。通过摄入不同剂量的酒精（浓度从 5%到 40%），12 周后建立 AFL 大鼠模型。测量血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、肿瘤坏死因子-α（TNF-α）、胰岛素和血糖水平，通过组织病理学变化确定，并用 Western blot 和免疫组化分别评估肝脏中脂肪分化相关蛋白（ADRP）的表达。结果表明，TFLC 治疗可降低血清 TG、TC、LDL-C、TNF-α、血糖和胰岛素水平，改善肝指数。形态学评价显示模型组大鼠发生严重脂肪变性，但 TFLC（200 和 400 mg/kg）治疗组可有效改善肝脂肪变性程度。模型组肝 ADRP 表达增加，而 TFLC 治疗组表达受抑制。这些结果表明，TFLC 对 AFL 大鼠具有保护作用；其机制可能涉及通过下调 AFL 大鼠肝脏 ADRP 的表达来调节血清脂质谱。
