Aquatic Sciences, South Australian Research and Development Institute, Henley Beach, South Australia, Australia.
Biotechnol Adv. 2010 Nov-Dec;28(6):706-14. doi: 10.1016/j.biotechadv.2010.05.018. Epub 2010 May 19.
Marine pest incursions can cause significant ongoing damage to aquaculture, biodiversity, fisheries habitat, infrastructure and social amenity. They represent a significant and ongoing economic burden. Marine pests can be introduced by several vectors including aquaculture, aquarium trading, commercial shipping, fishing, floating debris, mining activities and recreational boating. Despite the inherent risks, there is currently relatively little routine surveillance of marine pest species conducted in the majority of countries worldwide. Accurate and rapid identification of marine pest species is central to early detection and management. Traditional techniques (e.g. physical sampling and sorting), have limitations, which has motivated some progress towards the development of molecular diagnostic tools. This review provides a brief account of the techniques traditionally used for detection and describes developments in molecular-based methods for the detection and surveillance of marine pest species. Recent advances provide a platform for the development of practical, specific, sensitive and rapid diagnosis and surveillance tools for marine pests for use in effective prevention and control strategies.
海洋病虫害的入侵会对水产养殖业、生物多样性、渔业栖息地、基础设施和社会公共设施造成持续的重大破坏。它们是一个重大且持续存在的经济负担。海洋病虫害可以通过多种载体引入，包括水产养殖、水族馆交易、商业航运、渔业、漂浮残骸、采矿活动和休闲划船。尽管存在固有风险，但目前在世界上大多数国家，对海洋病虫害的常规监测相对较少。准确、快速地识别海洋病虫害是早期发现和管理的关键。传统技术（例如物理采样和分类）存在局限性，这促使人们在开发分子诊断工具方面取得了一些进展。本文简要介绍了传统的检测技术，并描述了基于分子的海洋病虫害检测和监测方法的进展。最近的进展为开发用于海洋病虫害的实用、特异、敏感和快速诊断和监测工具提供了一个平台，以便在有效的预防和控制策略中使用。
