细胞体积变化对体外培养的大鼠骨骼肌中谷氨酰胺和丙氨酸释放速率的影响。
Effects of changes in cell volume on the rates of glutamine and alanine release from rat skeletal muscle in vitro.
作者信息
Parry-Billings M, Bevan S J, Opara E, Newsholme E A
机构信息
Department of Biochemistry, University of Oxford, U.K.
出版信息
Biochem J. 1991 Jun 1;276 ( Pt 2)(Pt 2):559-61. doi: 10.1042/bj2760559.
The effect of changes in cell volume on the rates of release of glutamine and alanine from muscle and on the concentrations of these amino acids in muscle were investigated by using an isolated preparation of rat skeletal muscle incubated in the presence of hypo- and hyper-osmotic media. Changes in cell volume were associated with changes in the rates of release of glutamine and alanine from muscle: incubation in hypo-osmotic medium decreased the rates of release of glutamine and alanine, and incubation in hyperosmotic medium increased these rates. These changes were rapidly reversed by a change in osmoticity of the medium. Despite marked changes in cell volume, the concentrations of these amino acids in muscle were maintained. It is suggested that cell volume may play a role in the regulation of amino acid metabolism in skeletal muscle.
通过使用在低渗和高渗介质存在下孵育的大鼠骨骼肌分离制剂，研究了细胞体积变化对肌肉中谷氨酰胺和丙氨酸释放速率以及这些氨基酸在肌肉中浓度的影响。细胞体积的变化与肌肉中谷氨酰胺和丙氨酸释放速率的变化相关：在低渗介质中孵育会降低谷氨酰胺和丙氨酸的释放速率，而在高渗介质中孵育会增加这些速率。这些变化会随着介质渗透压的改变而迅速逆转。尽管细胞体积发生了显著变化，但肌肉中这些氨基酸的浓度仍保持不变。这表明细胞体积可能在骨骼肌氨基酸代谢的调节中起作用。
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