Postlethwaite A E, Seyer J M, Kang A H
Proc Natl Acad Sci U S A. 1978 Feb;75(2):871-5. doi: 10.1073/pnas.75.2.871.
The chemotactice response of human dermal fibroblasts of type I, II, and III human collagens and collagen-derived peptides was quantitated by an in vitro assay. All three native human collagens and constituent alpha chains can serve as chemoattractants for fibroblasts in vitro. When type I, II, and III collagens were digested by bacterial collagenase, the resulting peptides were also chemotactic. In addition, synthetic di- and tripeptides containing hydroxyproline were also chemotactic for fibroblasts. Since collagen is degraded and remodeled at sites of tissue injury and inflammation, these findings suggest that collagen and collagen-degradation peptides might function as chemotactic stimuli for fibroblasts in vivo and attract these cells to effect repair of damaged tissue.
通过体外试验对人I型、II型和III型胶原蛋白及胶原蛋白衍生肽刺激人皮肤成纤维细胞的趋化反应进行了定量分析。所有这三种天然人胶原蛋白及其组成的α链在体外均可作为成纤维细胞的化学引诱剂。当I型、II型和III型胶原蛋白被细菌胶原酶消化后,所产生的肽也具有趋化作用。此外,含羟脯氨酸的合成二肽和三肽对成纤维细胞也有趋化作用。由于胶原蛋白在组织损伤和炎症部位会发生降解和重塑,这些发现表明胶原蛋白和胶原蛋白降解肽在体内可能作为成纤维细胞的趋化刺激物,吸引这些细胞来修复受损组织。