胶原蛋白及胶原蛋白肽诱导人血单核细胞的趋化作用。
Collagen-and collagen peptide-induced chemotaxis of human blood monocytes.
作者信息
Postlethwaite A E, Kang A H
出版信息
J Exp Med. 1976 Jun 1;143(6):1299-307. doi: 10.1084/jem.143.6.1299.
Abstract
The ability of collagen and collagen-derived peptides to act as chemotactic stimuli was investigated by in vitro chemotaxis assays. Native human and chick skin collagen (type I) and alpha-chains obtained from purified chick skin collagen were each chemotactic for human peripheral blood monocytes. In addition, smaller peptides obtained either by digesting native collagen with bacterial collagenase or by degrading purified alpha-chains with cyanogen bromide or pepsin were also chemotactic for monocytes. In contrast, native collagen, alpha-chains, and smaller collagen-derived peptides were not chemotactic for human neutrophils. Since collagen is degraded at sites of tissue damage and inflammation, our findings suggest the possibility that such collagen-derived degradation products might directly serve as chemotactic stimuli for human peripheral blood monocytes in vivo.
摘要
通过体外趋化性分析研究了胶原蛋白及胶原蛋白衍生肽作为趋化刺激物的能力。天然人源和鸡皮胶原蛋白（I型）以及从纯化的鸡皮胶原蛋白中获得的α链对人外周血单核细胞均具有趋化作用。此外，通过用细菌胶原酶消化天然胶原蛋白或用溴化氰或胃蛋白酶降解纯化的α链获得的较小肽对单核细胞也具有趋化作用。相比之下，天然胶原蛋白、α链和较小的胶原蛋白衍生肽对人中性粒细胞没有趋化作用。由于胶原蛋白在组织损伤和炎症部位会被降解，我们的研究结果表明，这种胶原蛋白衍生的降解产物可能在体内直接作为人外周血单核细胞的趋化刺激物。
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本文引用的文献
1
Mechanisms of lysosomal enzyme release from leukocytes exposed to immune complexes and other particles.溶酶体酶从白细胞中释放的机制，白细胞暴露于免疫复合物和其他颗粒。
J Exp Med. 1971 Sep 1;134(3):149-65.
2
Characterization of the alpha-chains of chick skin collagen and the nature of the NH2-terminal cross-link region.鸡皮肤胶原蛋白α链的表征及氨基末端交联区域的性质
Biochemistry. 1969 Sep;8(9):3648-55. doi: 10.1021/bi00837a023.
3
Characterization of the cyanogen bromide peptides from the alpha-2 chain of chick skin collagen.鸡皮肤胶原蛋白α-2链溴化氰肽段的特性分析
Biochemistry. 1969 Aug;8(8):3200-4. doi: 10.1021/bi00836a011.
4
Characterization of the cyanogen bromide peptides from the alpha 1 chain of chick skin collagen.鸡皮肤胶原蛋白α1链溴化氰肽段的特性分析
Biochemistry. 1969 Apr;8(4):1506-14. doi: 10.1021/bi00832a029.
5
Leukotactic factor produced by sensitized lymphocytes.致敏淋巴细胞产生的白细胞趋化因子。
Science. 1969 Mar 7;163(3871):1079-81. doi: 10.1126/science.163.3871.1079.
6
Chemotoxis of mononuclear cells.单核细胞的化学毒性
J Exp Med. 1968 Nov 1;128(5):1201-21. doi: 10.1084/jem.128.5.1201.
7
A chemotactic factor for mononuclear leukocytes.一种单核白细胞趋化因子。
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8
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9
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