Research Unit of Biology and Genetics of Cancer, Haematological and Autoimmune Diseases, Faculty of Pharmacy of Monastir, Monastir 5000, Tunisia.
Clin Biochem. 2010 Sep;43(13-14):1085-9. doi: 10.1016/j.clinbiochem.2010.05.005. Epub 2010 May 21.
Our aim was to evaluate the contribution of tumor necrosis factor (TNF)-alpha -308G>A and interleukin (IL)-6 -174G>C gene promoter variants to the presence of coronary artery disease (CAD) in Tunisians.
Study subjects comprised 418 angiographically proven CAD patients and 406 age-, gender-, and ethnic origin-matched controls. Genotyping was performed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis.
There were no significant differences in the allelic distribution of TNF-alpha -308A (19.6% vs. 19.0%, P=0.73), and IL-6 -174C (15.6% vs. 14.3%, P=0.47) promoter polymorphisms between CAD patients and control subjects, respectively. In addition, single locus analysis revealed no differences in genotype frequencies between the two study groups, and the combined distribution of both genotypes did not differ significantly between controls and CAD patients (P>0.05).
There is no allelic or genotypic association of TNF-alpha -308G>A and IL-6 -174G>C promoter polymorphisms with CAD in Tunisians, thereby confirming an ethnic-selective contribution of both gene variants to CAD presence.
评估肿瘤坏死因子（TNF）-α -308G>A 和白细胞介素（IL）-6 -174G>C 基因启动子变异在突尼斯人群中对冠状动脉疾病（CAD）发生的影响。
研究对象包括 418 例经血管造影证实的 CAD 患者和 406 名年龄、性别和种族匹配的对照。采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析进行基因分型。
CAD 患者与对照组 TNF-α -308A（19.6% vs. 19.0%，P=0.73）和 IL-6 -174C（15.6% vs. 14.3%，P=0.47）启动子多态性的等位基因分布无显著差异。此外，单一位点分析显示两组间基因型频率无差异，且两种基因型的联合分布在对照组和 CAD 患者之间无显著差异（P>0.05）。
TNF-α -308G>A 和 IL-6 -174G>C 启动子多态性与突尼斯人 CAD 之间无等位基因或基因型关联，从而证实这两种基因变异在 CAD 发生中的种族选择性作用。
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