Hashad Ingy M, Nosseir Habiba, Shaban Gamal M, Abdel Rahman Mohamed F, Gad Mohamed Z
Clinical Biochemistry Unit, Faculty of Pharmacy and Biotechnology, the German University in Cairo, Cairo, Egypt.
National Heart Institute, Cairo, Egypt.
J Genet Eng Biotechnol. 2021 Sep 20;19(1):139. doi: 10.1186/s43141-021-00243-x.
Cardiovascular disease (CVD) remains the major cause of death worldwide. Most CVD can be prevented by addressing risk factors. Acute myocardial infarction (AMI) is an inflammatory disorder characterized by changes in several cytokines including the interleukins (ILs). Studies are running to evaluate the genetic variation in the inflammatory system and their influence on the risk factors for CVD aiming for future prevention of this global disease. The aim of the current study was too investigate the association of -174 (G/C) IL-6 polymorphism with the incidence of AMI in a representative sector of the Egyptian population and to examine the contribution of IL-6, as a biomarker, in the pathogenesis of AMI. Genotyping of -174 (G/C) IL-6 polymorphism was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) while IL-6 levels were assayed by ELISA.
The genotype distribution of -174 (G/C) IL-6 gene was not significantly different between the control subjects (GG 81.7%, GC 16.3%, CC 1.9%) and the AMI patients (GG 79%, GC 19%, CC 2%).The serum levels of IL-6 were significantly elevated in the AMI patients in comparison to the control subjects (P < 0.0001).
There is no significant association of -174(G/C) polymorphism in the promoter sequence of IL-6 and the incidence of AMI in the examined sample of Egyptian population. Elevated levels of serum IL-6 confirmed the relationship between inflammation and the incidence of AMI.
心血管疾病（CVD）仍然是全球主要的死亡原因。大多数心血管疾病可以通过控制危险因素来预防。急性心肌梗死（AMI）是一种炎症性疾病，其特征是包括白细胞介素（ILs）在内的多种细胞因子发生变化。目前正在进行研究，以评估炎症系统中的基因变异及其对心血管疾病危险因素的影响，旨在未来预防这种全球性疾病。本研究的目的是调查埃及人群代表性样本中IL-6基因-174（G/C）多态性与急性心肌梗死发病率的关联，并检验IL-6作为生物标志物在急性心肌梗死发病机制中的作用。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对IL-6基因-174（G/C）多态性进行基因分型，同时采用酶联免疫吸附测定（ELISA）法检测IL-6水平。
对照组（GG 81.7%，GC 16.3%，CC 1.9%）和急性心肌梗死患者组（GG 79%，GC 19%，CC 2%）之间IL-6基因-174（G/C）的基因型分布无显著差异。与对照组相比，急性心肌梗死患者的血清IL-6水平显著升高（P < 0.0001）。
在埃及人群的检测样本中，IL-6启动子序列中的-174（G/C）多态性与急性心肌梗死的发病率无显著关联。血清IL-6水平升高证实了炎症与急性心肌梗死发病率之间的关系。
