Animal Physiology Laboratory, Life Sciences Department, Sfax Faculty of Science, University of Sfax, BP1171, 3000 Sfax, Tunisia.
Ecotoxicol Environ Saf. 2011 May;74(4):811-9. doi: 10.1016/j.ecoenv.2010.11.007. Epub 2010 Dec 15.
Acute exposure to pesticides can cause hepatotoxicity. Our study pertains to the potential ability of selenium and/or vitamin E, used as nutritional supplements, to alleviate oxidative stress induced by dimethoate. Female Wistar rats were randomly divided into seven groups of six each: group I served as controls; group II received in their drinking water dimethoate (2 g L(-1)); group III received both dimethoate and selenium (0.5 mg/kg of diet); group IV was treated with dimethoate and vitamin E (100 mg/kg of diet); group V received dimethoate+selenium+vitamin E and groups VI and VII received either selenium or vitamin E. The exposure of rats to dimethoate for 30 days promoted oxidative stress with an increase in malondialdehyde and a decrease in glutathione and non-protein thiol levels. A decrease in glutathione peroxidase, superoxide dismutase and catalase activities was also observed. While, plasma transaminases, lactate dehydrogenase activities and bilirubin levels increased. Co-administration of selenium and/or vitamin E through diet improved the biochemical parameters cited above. Liver histological studies confirmed biochemical parameters and the beneficial roles of selenium and vitamin E.
急性暴露于杀虫剂会导致肝毒性。我们的研究涉及硒和/或维生素 E 作为营养补充剂，减轻乐果诱导的氧化应激的潜在能力。雌性 Wistar 大鼠随机分为七组，每组 6 只：第 I 组作为对照组；第 II 组在饮用水中摄入乐果（2 g/L）；第 III 组同时摄入乐果和硒（0.5 mg/kg 饮食）；第 IV 组用维生素 E（100 mg/kg 饮食）治疗；第 V 组接受乐果+硒+维生素 E；第 VI 和 VII 组分别接受硒或维生素 E。大鼠暴露于乐果 30 天会引发氧化应激，导致丙二醛增加，谷胱甘肽和非蛋白巯基水平降低。还观察到谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶活性降低。同时，血浆转氨酶、乳酸脱氢酶活性和胆红素水平升高。通过饮食联合使用硒和/或维生素 E 可以改善上述生化参数。肝组织学研究证实了硒和维生素 E 的生化参数和有益作用。
