用在不同实验动物中制备的超免疫血清对仙台病毒包膜抗原进行免疫电泳研究。
Immunoelectrophoretic study of Sendae virus envelope antigens with hyperimmune sera prepared in different laboratory animals.
作者信息
Ghyka G, Samuel I, Portocală R
出版信息
Virologie. 1978 Jan-Mar;29(1):23-8.
At similar HAI titers rabbit sera reveal a larger number of fractions (ten) in immunoelectrophoretic analysis of Sendai virus envelopes than do guinea pig sera, which only yield three distinct fractions. These differences are observed in both rocket and crossed immunoelectrophoresis, and are more marked in the latter. The number of fractions revealed does not differ in relation to the antigen used for hyperimmunization (intact Sendai virus, Triton-disrupted virus or envelope fractions extracted by Triton or Tween-ether). Solubilized Sendai virus envelopes obtained by Tween-ether or Triton treatment contain host antigens in their structure, the same as intact virus.
在相似的血凝抑制（HAI）滴度下，与豚鼠血清相比，兔血清在仙台病毒包膜的免疫电泳分析中显示出更多的组分（十个），豚鼠血清仅产生三个不同的组分。在火箭免疫电泳和交叉免疫电泳中均观察到这些差异，且在后者中更为明显。所显示的组分数量与用于超免疫的抗原（完整的仙台病毒、经 Triton 处理破坏的病毒或用 Triton 或吐温 - 乙醚提取的包膜组分）无关。通过吐温 - 乙醚或 Triton 处理获得的可溶解的仙台病毒包膜在其结构中含有宿主抗原，与完整病毒相同。
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