Lopez-Ribot J L, Casanova M, Martinez J P, Sentandreu R
Seccíon de Microbiología, Facultad de Farmacia, Universitat de València, Spain.
Infect Immun. 1991 Jul;59(7):2324-32. doi: 10.1128/iai.59.7.2324-2332.1991.
Cell surface hydrophobicity (CSH) of blastoconidia and blastoconidia bearing germ tubes of Candida albicans ATCC 26555 was monitored by assessing attachment of polystyrene microspheres to the cell surface, and we found that mature hyphae were significantly hydrophobic. Treatment of intact cells with low concentrations of beta-glucanase (Zymolyase 20T) or proteases abolished or significantly reduced attachment of latex beads to hyphae. This effect paralleled an obvious reduction in CSH of the entire cell population, as measured by an aqueous-hydrocarbon biphasic partitioning assay. Analysis of the cell wall material released by Zymolyase and adsorbed on polystyrene microspheres indicated that germ tube-specific cell wall proteins and mannoproteins with apparent molecular masses of 20 to 67 kDa may be responsible for the hydrophobicity of hyphae. Zymolyase released from blastoconidia cell walls a different set of proteins and mannoproteins that were able to adsorb to polystyrene microbeads. Such molecular species might in turn be responsible for the CSH exhibited by blastoconidium populations as determined by the biphasic partitioning assay, although these probably hydrophobic components can be masked on the surface of blastoconidia, as the latter had no or very few latex microspheres attached to their surfaces. Treatment of cells of both C. albicans morphologies with 2-mercaptoethanol released qualitatively distinct species of polystyrene-adsorbed proteins and mannoproteins from yeast and mycelial cells. These observations suggested that hydrophobic proteins and mannoproteins that could be associated with CSH are bound to the cell wall structure through diverse types of linkages.
通过评估聚苯乙烯微球与细胞表面的附着情况，监测白色念珠菌ATCC 26555的芽生孢子及带有芽管的芽生孢子的细胞表面疏水性（CSH），我们发现成熟菌丝具有显著的疏水性。用低浓度的β-葡聚糖酶（溶菌酶20T）或蛋白酶处理完整细胞，可消除或显著减少乳胶珠与菌丝的附着。这种效应与通过水-烃双相分配测定法测得的整个细胞群体CSH的明显降低相平行。对由溶菌酶释放并吸附在聚苯乙烯微球上的细胞壁物质的分析表明，表观分子量为20至67 kDa的芽管特异性细胞壁蛋白和甘露糖蛋白可能是菌丝疏水性的原因。溶菌酶从芽生孢子细胞壁释放出一组不同的能够吸附到聚苯乙烯微珠上的蛋白质和甘露糖蛋白。尽管这些可能的疏水成分在芽生孢子表面可能被掩盖，因为芽生孢子表面没有或只有很少的乳胶微球附着，但这些分子种类可能反过来是由双相分配测定法确定的芽生孢子群体所表现出的CSH的原因。用2-巯基乙醇处理白色念珠菌两种形态的细胞，从酵母细胞和菌丝细胞中释放出定性不同的聚苯乙烯吸附蛋白和甘露糖蛋白种类。这些观察结果表明，与CSH相关的疏水蛋白和甘露糖蛋白通过不同类型的连接与细胞壁结构结合。
