The Center for Disease Control and Prevention of Lanzhou Command, PLA, 509 Dong-Gang Dong Road, Chenguan District, Lanzhou 730020, PR China.
BMC Microbiol. 2010 May 28;10:157. doi: 10.1186/1471-2180-10-157.
Lyme disease is a multi-organ infection disease caused by Borrelia burgdorferi sensu lato. Lyme disease was first documented in north-east China in 1986. Since then more than 20 provinces in China were confirmed the existence of nature foci of Lyme disease. In the present study, a molecular epidemiological survey was conducted to investigate the presence of Borrelia burgdorferi sensu lato in rodents from Gansu Province for the first time.
A total of 140 rodents of 7 species were examined for Borrelia burgdorferi sensu lato. by nested-PCR and culture isolation. The overall infection rate was 22.86%. Two rodent species most frequently trapped were responsible for all positive. 3 strains were isolated from Apodemus agrarius, which belonged to B. garinii, 1 strain isolated from Rattus losea was identified as B. afzelii.
The study firstly showed the role of rodents in maintaining the pathogen of Lyme disease in the environment from Gansu Province and there existed at least two genotypes of Lyme disease spirochaetes in rodents.
莱姆病是一种由伯氏疏螺旋体引起的多器官感染性疾病。1986 年，莱姆病在中国东北首次被记录。此后，中国已有 20 多个省份证实存在莱姆病自然疫源地。本研究首次对甘肃省啮齿动物中伯氏疏螺旋体进行分子流行病学调查。
通过巢式 PCR 和培养分离法检测了 7 种共 140 只啮齿动物的伯氏疏螺旋体。总感染率为 22.86%。两种最常捕获的啮齿动物物种是所有阳性的罪魁祸首。从黑线姬鼠中分离出 3 株属于伯氏疏螺旋体格氏亚种的菌株，从大林姬鼠中分离出 1 株属于伯氏疏螺旋体阿氏亚种的菌株。
本研究首次表明，甘肃省啮齿动物在维持莱姆病病原体方面发挥了作用，并且在啮齿动物中存在至少两种莱姆病螺旋体基因型。
Zotero 插件