Department of Biochemistry and Microbiology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079-9502, USA.
Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):961-964. doi: 10.1099/ijs.0.021733-0. Epub 2010 May 28.
A novel halophilic bacterium of the genus Kangiella was isolated from a marine sponge collected from the Florida Keys, USA. Strain A79(T), an aerobic, Gram-negative, non-motile, rod-shaped bacterium, grew in 2-15 % (w/v) NaCl, at a temperature of 10-49 °C and at pH 4.5-10. Phylogenetic analysis placed strain A79(T) in the family Alcanivoraceae in the class Gammaproteobacteria. Strain A79(T) showed 98.5 % 16S rRNA gene sequence similarity to Kangiella japonica KMM 3899(T), 96.6 % similarity to Kangiella koreensis DSM 16069(T) and 95.6 % similarity to Kangiella aquimarina DSM 16071(T). The major cellular fatty acids were iso-C(11 : 0), iso-C(11 : 0) 3-OH, iso-C(15 : 0), iso-C(17 : 0) and iso-C(17 : 1)ω9c and the G+C content of the genomic DNA was 44.9 mol%. On the basis of physiological, chemotaxonomic and phylogenetic comparisons, strain A79(T) represents a novel species in the genus Kangiella, for which the name Kangiella spongicola sp. nov. is proposed. The type strain is A79(T) ( = ATCC BAA-2076(T) = DSM 23219(T)).
从美国佛罗里达州群岛采集的海绵中分离到一株嗜盐新的金氏菌属细菌。A79(T) 株是需氧、革兰氏阴性、非运动、杆状细菌，在 2-15%（w/v）NaCl、10-49°C 和 pH4.5-10 的条件下生长。系统发育分析将 A79(T)株置于γ变形菌纲的 Alcanivoraceae 科。A79(T)株与日本金氏菌 KMM 3899(T)的 16S rRNA 基因序列相似性为 98.5%，与韩国金氏菌 DSM 16069(T)的相似性为 96.6%，与海水金氏菌 DSM 16071(T)的相似性为 95.6%。主要细胞脂肪酸为 iso-C(11 : 0)、iso-C(11 : 0) 3-OH、iso-C(15 : 0)、iso-C(17 : 0) 和 iso-C(17 : 1)ω9c，基因组 DNA 的 G+C 含量为 44.9 mol%。根据生理、化学分类和系统发育比较，A79(T)株代表金氏菌属的一个新种，命名为海绵金氏菌 Kangiella spongicola sp. nov.。模式菌株为 A79(T)（ = ATCC BAA-2076(T) = DSM 23219(T)）。
