Wang Jiahua, Lu Ye, Nawaz Muhammad Z, Xu Jun
Institute of Oceanography, Shanghai Jiao Tong University, Shanghai, China.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Front Microbiol. 2018 Jun 7;9:1224. doi: 10.3389/fmicb.2018.01224. eCollection 2018.
The genus has recently been proposed within the family Kangiellaceae, belonging to order Oceanospirillales. Here, we report the complete genome sequence of a novel strain, FT102, which is the only species isolated from a deep sea sediment sample. Furthermore, gaps in the publicly available genome scaffold of DSM 16071 (NCBI Reference Sequence: NZ_ARFE00000000.1) were also filled using polymerase chain reaction (PCR) and Sanger sequencing. A comparative genomic analysis of five and 18 non- strains revealed insights into their metabolic potential. It was shown that low genomic redundancy and -lineage-specific gene loss are the key reasons behind the genome reduction in compared to that in any other free-living Oceanospirillales strain. The occurrence of relatively diverse and more frequent extracellular protease-coding genes along with the incomplete carbohydrate metabolic pathways in the genome suggests that has high extracellular protein degradation potential. Growth of strains has been observed using amino acids as the only carbon and nitrogen source and tends to increase with additional tryptone. Here, we propose that extracellular protein degradation and amino acid utilization are significant and prominent features of . Our study provides more insight into the genomic traits and proteolytic metabolic capabilities of .
该属最近在属于海洋螺菌目的康氏菌科中被提出。在此，我们报告了一株新菌株FT102的完整基因组序列，它是从深海沉积物样本中分离出的唯一的[属名]物种。此外，还使用聚合酶链反应（PCR）和桑格测序填补了DSM 16071公开可用基因组支架（NCBI参考序列：NZ_ARFE00000000.1）中的缺口。对5株[属名]菌株和18株非[属名]菌株进行的比较基因组分析揭示了它们的代谢潜力。结果表明，与任何其他自由生活的海洋螺菌目菌株相比，低基因组冗余和[属名]谱系特异性基因丢失是[属名]基因组减少的关键原因。基因组中相对多样且更频繁出现的细胞外蛋白酶编码基因以及不完整的碳水化合物代谢途径表明[属名]具有较高的细胞外蛋白质降解潜力。已观察到[属名]菌株以氨基酸作为唯一碳源和氮源生长，并且随着添加胰蛋白胨往往会增加。在此，我们提出细胞外蛋白质降解和氨基酸利用是[属名]的显著特征。我们的研究为[属名]的基因组特征和蛋白水解代谢能力提供了更多见解。
