UNAM, Facultad de Ingeniería, División de Ingeniería Eléctrica, Av Universidad 3000, Mexico, D., 04510, Mexico.
J Exp Biol. 2010 Jun 15;213(Pt 12):2154-64. doi: 10.1242/jeb.038240.
Previous results show that when unrestrained crayfish sleep, the electrical activity of the brain changes from multiple spikes (frequencies above 300 Hz) on a flat baseline to continuous slow waves at a frequency of 15-20 Hz. To study the temporal organization of such activity, we developed a tethered crayfish preparation that allows us to place electrodes on visually identified regions of the brain. Recording the electrical activity of different brain areas shows that when the animal is active (awake), slow waves are present only in the central complex. However, simultaneously with the animal becoming limp (sleeping), slow waves spread first to deuto- and then to protocerebrum, suggesting that the central complex of the crayfish brain acts as the sleep generator.
先前的研究结果表明，在不受限制的情况下，当小龙虾睡觉时，大脑的电活动会从平坦基线的多个尖峰（频率高于 300 Hz）转变为 15-20 Hz 的连续慢波。为了研究这种活动的时间组织，我们开发了一种系绳小龙虾准备，使我们能够在大脑的视觉识别区域放置电极。记录不同脑区的电活动表明，当动物活跃（清醒）时，只有在中央复合体中才存在慢波。然而，当动物变得松弛（入睡）时，慢波首先传播到后脑，然后传播到前脑，这表明小龙虾大脑的中央复合体充当了睡眠发生器。
