Wang S T, Pizzolato S, Demshar H P
Chemistry Section, Ontario Ministry of Health, Toronto, Canada.
J Anal Toxicol. 1991 Mar-Apr;15(2):66-70. doi: 10.1093/jat/15.2.66.
A simple dilution procedure for the determination of aluminum in human serum and urine is described. Serum and urine specimens were analyzed directly after dilution with 0.1% Triton X-100. The matrix-based calibration was used for quantitation with the use of Zeeman background atomic absorption spectrometry. The precision (CV) of the method was from 2.9 to 6.6% for serum concentrations ranging from 3.04 to 0.9 mumol/L. The accuracy of the method was vertified by analyzing U.S. National Bureau of Standards, RM 8419 bovine serum reference material, recovery studies, and comparison with the protein precipitation method. The Quebec Interlaboratory Comparison Program was used for the validation of the analytical performance. The mean value of aluminum in 63 healthy subjects was 0.06 mumol/L for serum and 0.24 mumol/L for urine.
本文描述了一种用于测定人血清和尿液中铝含量的简单稀释程序。血清和尿液标本用0.1% Triton X-100稀释后直接进行分析。采用基于基质的校准，通过塞曼背景原子吸收光谱法进行定量。对于血清浓度范围为3.04至0.9 μmol/L的情况，该方法的精密度（CV）为2.9%至6.6%。通过分析美国国家标准局RM 8419牛血清参考物质、回收率研究以及与蛋白质沉淀法进行比较，验证了该方法的准确性。使用魁北克实验室间比对计划对分析性能进行验证。63名健康受试者血清中铝的平均值为0.06 μmol/L，尿液中为0.24 μmol/L。
