Department of Food Science and Nutrition, Center for Food & Nutritional Genomics, Kyungpook National University, Daegu 702-701, Republic of Korea.
Chem Biol Interact. 2010 Aug 5;186(3):316-22. doi: 10.1016/j.cbi.2010.05.006. Epub 2010 May 16.
This study investigated the effects of fucoxanthin isolated from marine plant extracts on lipid metabolism and blood glucose concentration in high-fat diet fed C57BL/6N mice. The mice were divided into high-fat control (HFC; 20% fat, w/w), low-fucoxanthin (low-Fxn; HFC+0.05% Fxn, w/w) and high-fucoxanthin (high-Fxn; HFC+0.2% Fxn, w/w) groups. Fxn supplementation significantly lowered the concentration of plasma triglyceride with a concomitant increase of fecal lipids in comparison to the HFC group. Also, the hepatic lipid contents were significantly lowered in the Fxn supplemented groups which seemed to be due to the reduced activity of the hepatic lipogenic enzymes, glucose-6-phosphate dehydrogenase, malic enzyme, fatty acid synthase and phosphatidate phosphohydrolase and the enhanced activity of beta-oxidation. Plasma high-density lipoprotein cholesterol concentrations and its percentage were markedly elevated by Fxn supplementation. Activities of two key cholesterol regulating enzymes: 3-hydroxy-3-methylglutaryl coenzyme A reductase and acyl coenzyme A: cholesterol acyltransferase, were significantly suppressed by Fxn regardless of the dosage. Relative mRNA expressions of acyl-coA oxidase 1, palmitoyl (ACOX1) and peroxisome proliferators activated receptor alpha (PPARalpha) and gamma (PPARgamma) were significantly altered by Fxn supplementation in the liver. Fxn also lowered blood glucose and HbA(1c) levels along with plasma resistin and insulin concentrations. These results suggest that Fxn supplementation plays a beneficial role in not only regulating the plasma and hepatic lipids metabolism but also for blood glucose-lowering action in high-fat fed mice.
本研究旨在探讨从海洋植物提取物中分离出的岩藻黄质对高脂肪饮食喂养的 C57BL/6N 小鼠脂代谢和血糖浓度的影响。将小鼠分为高脂肪对照组（HFC；20%脂肪，w/w）、低岩藻黄质组（low-Fxn；HFC+0.05% Fxn，w/w）和高岩藻黄质组（high-Fxn；HFC+0.2% Fxn，w/w）。与 HFC 组相比，岩藻黄质补充剂显著降低了血浆甘油三酯的浓度，并伴随着粪便脂质的增加。此外，岩藻黄质补充剂组的肝脂质含量显著降低，这似乎是由于肝内脂肪生成酶葡萄糖-6-磷酸脱氢酶、苹果酸酶、脂肪酸合酶和磷酸酶的活性降低，以及β氧化的活性增强所致。血浆高密度脂蛋白胆固醇浓度及其百分比也因岩藻黄质补充而显著升高。无论剂量如何，岩藻黄质都能显著抑制两种关键胆固醇调节酶：3-羟-3-甲基戊二酰辅酶 A 还原酶和酰基辅酶 A：胆固醇酰基转移酶的活性。岩藻黄质补充还显著改变了肝脏中酰基辅酶 A 氧化酶 1、棕榈酰（ACOX1）和过氧化物酶体增殖物激活受体α（PPARalpha）和γ（PPARgamma）的相对 mRNA 表达。岩藻黄质还降低了血糖和 HbA1c 水平，以及血浆抵抗素和胰岛素浓度。这些结果表明，岩藻黄质补充不仅在调节血浆和肝脂质代谢方面发挥有益作用，而且对高脂肪喂养小鼠的降血糖作用也有影响。
