Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.
Am J Trop Med Hyg. 2010 Jun;82(6):1140-5. doi: 10.4269/ajtmh.2010.09-0778.
To determine the role of Bartonella species as causes of acute febrile illness in humans from Thailand, we used a novel strategy of co-cultivation of blood with eukaryotic cells and subsequent phylogenetic analysis of Bartonella-specific DNA products. Bartonella species were identified in 14 blood clots from febrile patients. Sequence analysis showed that more than one-half of the genotypes identified in human patients were similar or identical to homologous sequences identified in rodents from Asia and were closely related to B. elizabethae, B. rattimassiliensis, and B. tribocorum. The remaining genotypes belonged to B. henselae, B. vinsonii, and B. tamiae. Among the positive febrile patients, animal exposure was common: 36% reported owning either dogs or cats and 71% reported rat exposure during the 2 weeks before illness onset. The findings suggest that rodents are likely reservoirs for a substantial portion of cases of human Bartonella infections in Thailand.
为了确定巴尔通体物种在引起泰国人类急性发热性疾病中的作用，我们采用了一种新的策略，即与真核细胞共培养血液，并对巴尔通体特异性 DNA 产物进行系统发育分析。从发热患者的 14 个血凝块中鉴定出了巴尔通体物种。序列分析表明，在人类患者中鉴定出的一半以上基因型与亚洲啮齿动物中鉴定出的同源序列相似或相同，与 B. elizabethae、B. rattimassiliensis 和 B. tribocorum 密切相关。其余基因型属于 B. henselae、B. vinsonii 和 B. tamiae。在阳性发热患者中，动物接触很常见：36%的患者报告拥有狗或猫，71%的患者在发病前 2 周内报告接触过老鼠。这些发现表明，啮齿动物可能是泰国人类巴尔通体感染的大部分病例的宿主。
