Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
Biol Pharm Bull. 2010;33(6):983-7. doi: 10.1248/bpb.33.983.
The mycelia of the edible mushroom Lentinula edodes can be cultured in solid medium containing lignin, and the hot-water extracts (L.E.M.) is commercially available as a nutritional supplement. During the cultivation, phenolic compounds, such as syringic acid and vanillic acid, were produced by lignin-degrading peroxidase secreted from L. edodes mycelia. Since these compounds have radical scavenging activity, we examined their protective effect on oxidative stress in mice with CCl(4)-induced liver injury. We examined the hepatoprotective effect of syringic acid and vanillic acid on CCl(4)-induced chronic liver injury in mice. The injection of CCl(4) into the peritoneal cavity caused an increase in the serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. The intravenous administration of syringic acid and vanillic acid significantly decreased the levels of the transaminases. Four weeks of CCl(4) treatment caused a sufficiently excessive deposition of collagen fibrils. An examination of Azan-stained liver sections revealed that syringic acid and vanillic acid obviously suppressed collagen accumulation and significantly decreased the hepatic hydroxyproline content, which is the quantitative marker of fibrosis. Both of these compounds inhibited the activation of cultured hepatic stellate cells, which play a central role in liver fibrogenesis, and maintained hepatocyte viability. These data suggest that the administration of syringic acid and vanillic acid could suppress hepatic fibrosis in chronic liver injury.
香菇菌丝体可在含有木质素的固体培养基中培养，香菇热水提取物（L.E.M.）可作为营养补充剂商业化。在培养过程中，木质素降解过氧化物酶从香菇菌丝体中分泌出来，产生了酚类化合物，如丁香酸和香草酸。由于这些化合物具有清除自由基的活性，我们研究了它们对 CCl4 诱导的肝损伤小鼠氧化应激的保护作用。我们研究了丁香酸和香草酸对 CCl4 诱导的慢性肝损伤小鼠的保肝作用。腹腔注射 CCl4 会导致血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平升高。静脉注射丁香酸和香草酸可显著降低转氨酶水平。4 周的 CCl4 处理会导致胶原纤维过度沉积。阿赞染色的肝切片检查显示，丁香酸和香草酸明显抑制胶原积累，并显著降低肝羟脯氨酸含量，这是纤维化的定量标志物。这两种化合物均抑制了在肝纤维化中起核心作用的培养肝星状细胞的活化，并维持了肝细胞的活力。这些数据表明，丁香酸和香草酸的给药可能抑制慢性肝损伤中的肝纤维化。
