Division of Virology, National Veterinary Institute, Technical University of Denmark, Lindholm, 4771 Kalvehave, Denmark.
Vet Rec. 2010 Jun 5;166(23):714-8. doi: 10.1136/vr.b4847.
Following the first ever case of bluetongue in Denmark during late 2007, further outbreaks were observed in Denmark during 2008, despite vaccination against bluetongue virus (BTV) serotype 8 (BTV-8) in the southern part of the country. In total, 15 separate outbreaks of infection were identified, mostly as a result of clinical suspicions but also because of surveillance of bulk milk samples. These outbreaks led to extensions of the original vaccination zone planned for 2008. Blood samples from clinical suspects were analysed using ELISA and real-time RT-PCR assays for the presence of anti-BTV antibodies and viral RNA, respectively. A newly infected calf from the primary outbreak in 2008 was studied for a period of three months, during which time it seroconverted to BTV, but the presence of viral RNA in its blood was maintained throughout this time. Each outbreak was caused by BTV-8, as determined by a serotype-specific real-time RT-PCR assay. Furthermore, the nucleotide sequence of a portion of segment 2 of the viral RNA (encoding the outer capsid protein VP2) from the samples analysed was identical to the BTV-8 segment 2 that circulated in the Netherlands during 2006.
2007 年末，丹麦首次出现蓝舌病病例，尽管该国南部对 8 型蓝舌病病毒（BTV-8）进行了疫苗接种，但 2008 年丹麦仍出现了更多的疫情。总共有 15 起单独的感染爆发，主要是由于临床怀疑，但也因为对散装牛奶样本的监测。这些疫情导致了原定于 2008 年的疫苗接种区的扩大。使用 ELISA 和实时 RT-PCR 检测从临床疑似病例中采集的血液样本，以检测抗 BTV 抗体和病毒 RNA 的存在。对 2008 年首次爆发时的一头新感染小牛进行了为期三个月的研究，在此期间它对 BTV 血清转化，但在此期间其血液中的病毒 RNA 一直存在。每个疫情都是由 BTV-8 引起的，这是通过一种血清型特异性实时 RT-PCR 检测确定的。此外，从分析样本中提取的病毒 RNA （编码外壳蛋白 VP2）的第 2 节的一部分核苷酸序列与 2006 年在荷兰流行的 BTV-8 节 2 完全相同。
