Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Nat Chem Biol. 2010 Jul;6(7):515-7. doi: 10.1038/nchembio.372. Epub 2010 Jun 6.
Coenzyme Q (Q) is an electron transporter in the respiratory chain and a lipid-soluble antioxidant that decreases in humans with age. Here we show that 4-nitrobenzoate inhibited 4-hydroxybenzoate:polyprenyl transferase (Coq2) in a competitive manner and dose-dependently decreased Q in mammalian cells without accumulation of Q intermediates. As 4-nitrobenzoate neither interfered with mitochondrial respiration nor induced oxidative stress, it should prove a valuable tool for studies on both Q deficiency and Q supplementation.
辅酶 Q（Q）是呼吸链中的电子转运体，也是一种脂溶性抗氧化剂，随着人体年龄的增长而减少。在这里，我们发现 4-硝基苯甲酸以竞争性方式抑制 4-羟基苯甲酸：多萜基转移酶（Coq2），并在不积累 Q 中间体的情况下，剂量依赖性地降低哺乳动物细胞中的 Q。由于 4-硝基苯甲酸既不干扰线粒体呼吸，也不诱导氧化应激，因此它应该成为研究 Q 缺乏和 Q 补充的有价值的工具。
