Department of Plant Science, McGill University, 21111 Lakeshore Road, Sainte-Anne-de-Bellevue, QC H9X 3V9, Canada.
J Agric Food Chem. 2010 Jul 14;58(13):7604-15. doi: 10.1021/jf101029a.
Sclerotia are vegetative structures that play a major role in survival of fungi under adverse conditions. The sclerotia of the plant pathogen Rhizoctonia solani AG2-2 IIIB exude liquid brown droplets that were evaluated for their bioactivity and toxicity against microorganisms and plant species. Also, their metabolic composition was analyzed by integrating Fourier transform ion cyclotron resonance-mass spectrometry (FT-ICR/MS), gas chromatography-MS (GC/MS), and proton nuclear magnetic resonance ((1)H NMR) spectroscopy. The results showed that exudates are complex mixtures composed of phenolics (17.40%), carboxylic acids (12.79%), carbohydrates (6.08%), fatty acids (3.78%), and amino acids (3.47%). The presence of such metabolites contributed to their antifungal and phytotoxic activities. The biological interpretation of the results highly suggests that the exudates not only have multiple roles in fungal physiology but also are a potential bioactive source with moderate toxicity. Our findings show with certainty that the integration of different analytical platforms is a powerful approach for extracting the maximum and reliable information on the metabolic composition of complex biological samples.
菌核是真菌在不利条件下生存的营养结构，起着重要作用。植物病原菌立枯丝核菌 AG2-2 IIIB 的菌核会渗出褐色的液体液滴，我们评估了这些液滴的生物活性和对微生物及植物物种的毒性。此外，我们还通过整合傅里叶变换离子回旋共振质谱（FT-ICR/MS）、气相色谱-质谱联用（GC/MS）和质子核磁共振波谱（1H NMR）分析了其代谢成分。结果表明，渗出物是由酚类物质（17.40%）、羧酸（12.79%）、碳水化合物（6.08%）、脂肪酸（3.78%）和氨基酸（3.47%）组成的复杂混合物。这些代谢产物的存在导致了其抗真菌和植物毒性活性。结果的生物学解释高度表明，渗出物不仅在真菌生理学中具有多种作用，而且还是一种具有中等毒性的潜在生物活性来源。我们的研究结果明确表明，整合不同的分析平台是提取复杂生物样品代谢成分的最大和可靠信息的有力方法。
