Wang Haining, Yang Xiaohe, Wei Songhong, Wang Yan
Department of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154007, China.
Pathogens. 2021 Mar 18;10(3):364. doi: 10.3390/pathogens10030364.
Rice false smut (RFS) disease, which is caused by , has been widespread all over the world in recent years, causing irreversible losses. Under artificial culture conditions, exudates will appear on colonies of during the growth of the hyphae. Exudation of droplets is a common feature in many fungi, but the functions of exudates are undetermined. As the executors of life functions, proteins can intuitively reflect the functions of exudates. Shotgun proteomics were used in this study. A total of 650 proteins were identified in the exudate of , and the raw data were made available via ProteomeXchange with the identifier PXD019861. There were 57 subcategories and 167 pathways annotated with Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, respectively. Through protein-protein interaction (PPI) network analysis, it was found that 20 proteins participated in the biosynthesis of secondary metabolites. Two separate PPI analyses were performed for carbon metabolism and microbial metabolism in diverse environments. After comparing and annotating the functions of proteins of the exudate, it was speculated that the exudate was involved in the construction and remodeling of the fungal cell wall. Pathogenicity, sporulation, and antioxidant effects might all be affected by the exudate.
稻曲病由[未提及具体病原体]引起，近年来在全球广泛传播，造成了不可逆转的损失。在人工培养条件下，[未提及具体真菌名称]菌丝生长过程中菌落上会出现渗出物。液滴渗出是许多真菌的常见特征，但渗出物的功能尚未确定。蛋白质作为生命功能的执行者，能够直观地反映渗出物的功能。本研究采用鸟枪法蛋白质组学。在[未提及具体真菌名称]的渗出物中总共鉴定出650种蛋白质，原始数据已通过ProteomeXchange以标识符PXD019861提供。分别通过基因本体论（GO）分类和京都基因与基因组百科全书（KEGG）通路分析注释了57个亚类和167条通路。通过蛋白质-蛋白质相互作用（PPI）网络分析发现，有20种蛋白质参与次生代谢产物的生物合成。针对碳代谢和不同环境下的微生物代谢进行了两次独立的PPI分析。在对渗出物蛋白质的功能进行比较和注释后，推测渗出物参与了真菌细胞壁的构建和重塑。致病性、孢子形成和抗氧化作用可能都受到渗出物的影响。
