Department of Pathology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.
Int Urol Nephrol. 2011 Mar;43(1):125-9. doi: 10.1007/s11255-010-9779-7. Epub 2010 Jun 10.
This study was aimed at evaluating the association between tumor vascularity and other known prognostic factors in different histopathological subtypes of renal cell carcinoma (RCC). In 41 cases of RCC, immunohistochemistry was performed using anti-CD34 antibody and Ki-67 (MIB-1). Microvessel density was estimated in an area of 0.23 mm(2) representative of the highest microvessel density ("hot spot"). The microvessel area (MVA) was expressed as percentage of total tumor area occupied by vessels. Endothelial proliferation index (EPI) was determined from sections stained by Ki-67. The relationship between MVD, MVA, EPI and prognostic parameters of RCC like stage, grade and tumor cells proliferation index (TPI) was evaluated. The tumor MVA increased significantly with the higher stage of the tumor (P < 0.001) and increase in TPI (P < 0.05). The MVD, however, did not show significant correlation with grade and stage of the tumor. Microvessel area appears to be a better prognostic marker for RCC when compared to microvessel density. The increase in MVA, without increase in MVD, with increasing stage may be due to the development of larger diameter vascular channels in higher-stage tumors, and this is thought to facilitate metastasis.
本研究旨在评估肿瘤血管生成与肾细胞癌（RCC）不同组织病理学亚型中其他已知预后因素之间的关系。在 41 例 RCC 中，使用抗 CD34 抗体和 Ki-67（MIB-1）进行免疫组织化学染色。微血管密度（MVD）在代表最高微血管密度的 0.23mm²区域（“热点”）中进行评估。微血管面积（MVA）表示血管占肿瘤总面积的百分比。从 Ki-67 染色的切片中确定内皮细胞增殖指数（EPI）。评估了 MVD、MVA、EPI 与 RCC 的预后参数（如分期、分级和肿瘤细胞增殖指数（TPI））之间的关系。肿瘤 MVA 随着肿瘤分期的升高而显著增加（P<0.001），TPI 也随之增加（P<0.05）。然而，MVD 与肿瘤的分级和分期没有显著相关性。与微血管密度相比，MVA 似乎是 RCC 更好的预后标志物。随着分期的增加，MVA 增加而 MVD 没有增加，这可能是由于较高分期肿瘤中较大直径血管通道的发展，这被认为有助于转移。
