根管冲洗剂抗真菌活性的比较研究
A comparative study of antifungal activity of endodontic irrigants.
作者信息
Mohammadi Zahed, Asgary Saeed
机构信息
Iranian National Elites Foundation, Tehran, Iran;
Iranian Center for Endodontic Research, Research Institute of Dental sciences, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Iran Endod J. 2015;10(2):144-7. Epub 2015 Mar 18.
INTRODUCTION
The purpose of this in vitro study was to assess the antifungal activity of final canal rinse with either three concentrations of sodium hypochlorite (NaOCl) (0.5, 2.6 and 6%), two concentrations of chlorhexidine (CHX) (2% and 0.2%), MTAD, Tetraclean, Hypoclean and Chlor-Xtra on Candida albicans (C. albicans) in a human tooth model.
METHODS AND MATERIALS
Two hundred and thirty five extracted human maxillary central and lateral incisors were used in this study. Teeth were randomly divided into nine test groups (n=25) and positive and a negative control groups (n=5). After cleaning and shaping, teeth were contaminated with C. albicans and incubated for 72 h. The irrigation solution in nine experimental groups included: 6% NaOCl, 2.6% NaOCl, 0.5% NaOCl, 2% CHX, 0.2% CHX, MTAD, Tetraclean, Hypoclean and Chlor-Xtra. After culturing on Sabouraud 4% dextrose agar, colony-forming units (CFU) were counted.
RESULTS
6% NaOCl, 2% CHX and Chlor-Xtra were equally effective (P>0.05) and significantly superior to MTAD and Tetraclean (P<0.05). In addition, the effectiveness of Tetraclean and MTAD was significantly less than Hypoclean, NaOCl at all concentrations (6% 2.6% and 0.5%), MTAD and 0.2% CHX (P<0.05). Furthermore, Tetraclean was significantly more effective than MTAD (P<0.05).
CONCLUSION
Antifungal activity of 6% NaOCl, Chlor-Xtra and 2% CHX was significantly greater than 2.6% NaOCl, 0.5% NaOCl, MTAD, 0.2% CHX and Tetraclean.
引言
本体外研究的目的是在人体牙齿模型中评估三种浓度的次氯酸钠（NaOCl）（0.5%、2.6%和6%）、两种浓度的洗必泰（CHX）（2%和0.2%）、MTAD、Tetraclean、Hypoclean和Chlor-Xtra作为最终根管冲洗液对白色念珠菌的抗真菌活性。
方法和材料
本研究使用了235颗拔除的人类上颌中切牙和侧切牙。牙齿被随机分为九个试验组（n = 25）以及阳性和阴性对照组（n = 5）。在清洁和预备根管后，牙齿被白色念珠菌污染并孵育72小时。九个实验组的冲洗液包括：6% NaOCl、2.6% NaOCl、0.5% NaOCl、2% CHX、0.2% CHX、MTAD、Tetraclean、Hypoclean和Chlor-Xtra。在沙氏4%葡萄糖琼脂上培养后，计算菌落形成单位（CFU）。
结果
6% NaOCl、2% CHX和Chlor-Xtra的效果相同（P>0.05），且显著优于MTAD和Tetraclean（P<0.05）。此外，Tetraclean和MTAD的效果显著低于Hypoclean、所有浓度的NaOCl（6%、2.6%和0.5%）、MTAD和0.2% CHX（P<0.05）。此外，Tetraclean比MTAD的效果显著更好（P<0.05）。
结论
6% NaOCl、Chlor-Xtra和2% CHX的抗真菌活性显著高于2.6% NaOCl、0.5% NaOCl、MTAD、0.2% CHX和Tetraclean。
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