Giardino Luciano, Ambu Emanule, Savoldi Enrico, Rimondini Roberto, Cassanelli Clara, Debbia Eugenio A
Department of Periodontology, Dental School, University of Brescia, Brescia, Italy.
J Endod. 2007 Jul;33(7):852-5. doi: 10.1016/j.joen.2007.02.012. Epub 2007 Apr 2.
The aim of this study was to compare the antimicrobial efficacy of 5.25% NaOCl, BioPure MTAD (Dentsply Tulsa Dental, Johnson City, TN), and Tetraclean (Ogna Laboratori Farmaceutici, Milano, Italy) against Enterococcus faecalis biofilm generated on cellulose nitrate membrane filters. After incubation, the membrane filters were transferred into tubes containing 5 mL of the selected antimicrobial solution test agent or NaCl 0.9% (positive control) and incubated for 5, 30, and 60 minutes at 20 degrees C. After each period of time, the test agents were vortexed for 60 seconds to resuspend the microorganisms. Ten-fold serial dilutions were generated in reduced transport fluid. Each dilution was plated onto a brain heart infusion plates. The plates were then incubated for 48 hours in an aerobic atmosphere at 37 degrees C and colony-forming units per membrane was calculated. Statistical analysis showed that only 5.25% NaOCl can disgregate and remove the biofilm at every time; however, treatment with Tetraclean caused a high degree of biofilm disgregation in every considered time intervals as compared with MTAD (T5 p < 0.05, T30 p < 0.01, and T60 p < 0.001).
本研究的目的是比较5.25%次氯酸钠、BioPure MTAD（登士柏塔尔萨牙科公司，田纳西州约翰逊城）和Tetraclean（奥尼亚制药实验室，意大利米兰）对硝酸纤维素膜过滤器上生成的粪肠球菌生物膜的抗菌效果。培养后，将膜过滤器转移到含有5 mL选定抗菌溶液测试剂或0.9%氯化钠（阳性对照）的试管中，并在20℃下孵育5、30和60分钟。在每个时间段后，将测试剂涡旋60秒以重新悬浮微生物。在稀释转运液中进行10倍系列稀释。将每种稀释液接种到脑心浸液平板上。然后将平板在37℃需氧气氛中孵育48小时，并计算每个膜上的菌落形成单位。统计分析表明，只有5.25%次氯酸钠在每个时间点都能分解并去除生物膜；然而，与MTAD相比，在每个考虑的时间间隔内，用Tetraclean处理都会导致高度的生物膜分解（T5时p<0.05，T30时p<0.01，T60时p<0.001）。
