U.S. Food and Drug Administration, 5100 Paint Branch Parkway HFS-711, College Park, Maryland 20740, USA.
J Food Prot. 2010 Jun;73(6):1016-22. doi: 10.4315/0362-028x-73.6.1016.
Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F chromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.
阪崎肠杆菌可引起严重疾病，在某些情况下可导致婴儿死亡。受阪崎肠杆菌污染的配方粉已被证实是几起涉及婴儿的暴发事件的潜在原因。本研究描述了一种从配方粉中分离和检测阪崎肠杆菌的方法。它结合了 Taqman 实时 PCR、 Brilliance 阪崎肠杆菌和 R&F 显色琼脂，以及 RAPID ID 32E 生化测试。该方法可根据配方粉中阪崎肠杆菌和其他微生物的数量和应激状态，在 1 至 2 天内提供便捷的分析。实时 PCR 具有双重应用，包括阪崎肠杆菌的筛查和培养确认。
