Shimizu H, Mitsuda T, Fujita S, Yokota S
Department of Paediatrics, Yokohama City University School of Medicine, Japan.
Arch Dis Child. 1991 Jun;66(6):718-21. doi: 10.1136/adc.66.6.718.
To investigate the infectivity of hepatitis B virus (HBV) from mothers to their newborn offspring, HBV-DNA in plasma and peripheral mononuclear cells from 28 antihepatitis Be positive, hepatitis B surface antigen positive carrier mothers was examined by a highly sensitive polymerase chain reaction/Southern hybridisation technique. HBV specific DNA was detected in three maternal mononuclear cell samples, but was absent in plasma. Two of four infants born to the three mothers with HBV-DNA positive mononuclear cells developed acute or fulminant hepatitis within three months after birth. Two infants were effectively prevented from infection with HBV by combined hepatitis B immunoglobulin/HBV vaccine administration. The 25 infants born to the HBV-DNA negative mothers were free of HBV infection within the next seven months to 3.5 years. These results suggest that latent infection with HBV in maternal mononuclear cells is responsible for perinatal HBV infection.
为研究乙型肝炎病毒（HBV）从母亲到其新生儿后代的传染性，采用高灵敏度聚合酶链反应/ Southern杂交技术检测了28例抗-HBe阳性、乙肝表面抗原阳性携带者母亲血浆和外周血单个核细胞中的HBV-DNA。在3例母亲的单个核细胞样本中检测到HBV特异性DNA，但血浆中未检测到。3例HBV-DNA阳性单个核细胞母亲所生的4例婴儿中有2例在出生后3个月内发生急性或暴发性肝炎。通过联合使用乙型肝炎免疫球蛋白/ HBV疫苗，有效预防了另外2例婴儿感染HBV。25例HBV-DNA阴性母亲所生的婴儿在接下来的7个月至3.5年内未感染HBV。这些结果表明，母亲单个核细胞中HBV的潜伏感染是围产期HBV感染的原因。
