Jeamah Archig, Senarat Sinlapachai, Kong-Oh Suparat, Sudtongkong Chanyut, Wirachwong Porntep, Charoenphon Natthawut, Kawjaeng Nontawat, Kosiyachinda Pahol, Kenthao Anan, Boonyoung Piyakorn
Department of Marine Science and Environment, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang 92150, Thailand.
Division of Biological Science, Faculty of Science, Prince of Songkhla University, Songkhla 90110, Thailand.
Trop Life Sci Res. 2023 Sep;34(3):113-127. doi: 10.21315/tlsr2023.34.3.6. Epub 2023 Sep 30.
Hematological evaluation of fish is essential to the assessment of their physiological status. This study describes the morphometric analysis and comparison of blood cell characteristics in sp., Cuvier, 1829 and (De Vis, 1884). The species were collected at two locations off the coast of Trang Province, Thailand. A comparative hematological evaluation was made to assess the effects of environmental conditions on the blood of the fish. Ten individuals of each species were collected from a seagrass bed at Libong Island, where human activities are increasing, and from a secluded sandy beach. Their blood samples were analysed using the blood smear technique. Erythrocytes of all the studied fishes were either elliptical or oval. The morphometric data from both locations showed that erythrocytes were of similar size, except for those of sp. Fish from both stations showed several types of leukocytes, including neutrophils and lymphocytes.The highest proportion of leukocytes was made up of lymphocytes, followed by neutrophils. However, monocytes were only observed in fish from Libong Island and the erythrocytic nuclei of fish collected from Libong Island were both reniform and lobate. Our results show the potential of hematological evaluation as an early warning signal of environmental impacts on aquatic animals. The determination of baseline parameters could provide a tool for the monitoring of environmental quality.
对鱼类进行血液学评估对于了解它们的生理状态至关重要。本研究描述了1829年命名的 种和1884年命名的 种的血细胞形态分析及特征比较。这些物种采自泰国董里府海岸外的两个地点。通过比较血液学评估来评估环境条件对鱼类血液的影响。每个物种从人类活动日益增加的利邦岛的海草床和一个偏僻的沙滩各采集10个个体。使用血液涂片技术对它们的血液样本进行分析。所有研究鱼类的红细胞均为椭圆形或卵形。两个地点的形态学数据表明，除 种的红细胞外，其他鱼类的红细胞大小相似。两个采样点的鱼类均显示出几种类型的白细胞，包括中性粒细胞和淋巴细胞。白细胞中比例最高的是淋巴细胞，其次是中性粒细胞。然而，单核细胞仅在利邦岛的鱼类中观察到，并且从利邦岛采集的鱼类的红细胞核既有肾形的也有叶状的。我们的结果表明，血液学评估有潜力作为环境对水生动物影响的早期预警信号。确定基线参数可为监测环境质量提供一种工具。
