LE Gao-Zhong, Dong Xiang-Yu, Shen Yang, Chen Yong-Qian, Lu Jin-Ping
Department of Pediatrics, Second Affiliated Hospital of Lanzhou University, Lanzhou 730030, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2010 Jun;12(6):440-3.
To study erythrocyte oxidative stress status and its association with left to right shunt congenital heart disease (CHD) in children.
A total of 31 children with left to right shunt CHD were enrolled, including 7 cases of atrial septal defect (ASD), 12 ventricular septal defect (VSD), 4 patent ductus arteriosus (PDA), 6 patent foramen ovale (PFO), and 2 complete endocardial cushion defect. Twenty healthy age-matched (1 month to 3 years old) children severed as the control group. The contents of superoxide dismutase (SOD) and malonaldehyde (MDA) in erythrocytes were determined using ELISA. ESR was measured by Westergen. PaO(2) and PaCO(2) were measured by Blood Gas Analyzer (GEM Premier 3000).
The MDA content in erythrocytes in the CHD group was significantly higher, in contrast, SOD content was significantly lower than that in the control group (P<0.05). The CHD children with heart failure had more decreased SOD and more increased MDA contents compared with the control group (P<0.01). The SOD level was the highest in the PFO group and was the lowest in the complete endocardial cushion defect group. The SOD level in the PFO group was significantly higher than that in the ASD, VSD and complete endocardial cushion defect groups (P<0.05). The MDA level was the highest in the VSD group and was the lowest in the complete endocardial cushion defect group. There were significant differences in the MDA level among CHD subgroups (P<0.05). The ESR was negatively correlated to the SOD level (r=-0.191, P<0.05), while positively correlated to PaO(2) level in CHD children (r=0.216, P<0.05). There was a negative correlation between SOD and MDA levels (r=-0.312, P<0.05).
Oxidative stress exists in children with left to right shunt CHD. The SOD and MDA contents in erythrocytes can be used as markers for the assessment of severity of the disease.
研究儿童左向右分流型先天性心脏病（CHD）红细胞氧化应激状态及其相关性。
纳入31例左向右分流型CHD患儿，包括7例房间隔缺损（ASD）、12例室间隔缺损（VSD）、4例动脉导管未闭（PDA）、6例卵圆孔未闭（PFO）和2例完全性心内膜垫缺损。选取20例年龄匹配（1个月至3岁）的健康儿童作为对照组。采用ELISA法测定红细胞中超氧化物歧化酶（SOD）和丙二醛（MDA）的含量。采用魏氏法测定血沉（ESR）。采用血气分析仪（GEM Premier 3000）测定动脉血氧分压（PaO₂）和动脉血二氧化碳分压（PaCO₂）。
CHD组红细胞中MDA含量显著高于对照组，而SOD含量显著低于对照组（P<0.05）。与对照组相比，合并心力衰竭的CHD患儿SOD降低更明显，MDA升高更明显（P<0.01）。PFO组SOD水平最高，完全性心内膜垫缺损组最低。PFO组SOD水平显著高于ASD、VSD和完全性心内膜垫缺损组（P<0.05）。VSD组MDA水平最高，完全性心内膜垫缺损组最低。CHD各亚组间MDA水平差异有统计学意义（P<0.05）。CHD患儿ESR与SOD水平呈负相关（r=-0.191，P<0.05），与PaO₂水平呈正相关（r=0.216，P<0.05）。SOD与MDA水平呈负相关（r=-0.312，P<0.05）。
左向右分流型CHD患儿存在氧化应激。红细胞中SOD和MDA含量可作为评估该病严重程度的指标。
