Vanreusel Inne, Vermeulen Dorien, Goovaerts Inge, Stoop Tibor, Ectors Bert, Cornelis Jacky, Hens Wendy, de Bliek Erwin, Heuten Hilde, Van Craenenbroeck Emeline M, Van Berendoncks An, Segers Vincent F M, Briedé Jacob J
Department of Cardiology, Antwerp University Hospital, 2650 Edegem, Belgium.
Research Group Cardiovascular Diseases, GENCOR, University of Antwerp, 2000 Antwerp, Belgium.
Antioxidants (Basel). 2022 Nov 30;11(12):2369. doi: 10.3390/antiox11122369.
Oxidative stress is an important pathophysiological mechanism in the development of numerous cardiovascular disorders, but few studies have examined the levels of oxidative stress in adults with congenital heart disease (CHD). The objective of this study was to investigate oxidative stress levels in adults with CHD and the association with inflammation, exercise capacity and endothelial function. To this end, 36 adults with different types of CHD and 36 age- and gender-matched healthy controls were enrolled. Blood cell counts, hs-CRP, NT-proBNP, fasting glucose, cholesterol levels, iron saturation and folic acid concentrations were determined in venous blood samples. Levels of superoxide anion radical in whole blood were determined using electron paramagnetic resonance spectroscopy in combination with the spin probe CMH. Physical activity was assessed with the IPAQ-SF questionnaire. Vascular function assessment (EndoPAT) and cardiopulmonary exercise testing were performed in the patient group. Superoxide anion radical levels were not statistically significantly different between adults with CHD and the matched controls. Moreover, oxidative stress did not correlate with inflammation, or with endothelial function or cardiorespiratory fitness in CHD; however, a significant negative correlation with iron saturation was observed. Overall, whole blood superoxide anion radical levels in adults with CHD were not elevated, but iron levels seem to play a more important role in oxidative stress mechanisms in CHD than in healthy controls. More research will be needed to improve our understanding of the underlying pathophysiology of CHD.
氧化应激是众多心血管疾病发生发展过程中的一种重要病理生理机制，但很少有研究检测先天性心脏病（CHD）成人患者的氧化应激水平。本研究的目的是调查CHD成人患者的氧化应激水平及其与炎症、运动能力和内皮功能的关系。为此，招募了36例患有不同类型CHD的成人患者以及36例年龄和性别匹配的健康对照者。测定静脉血样本中的血细胞计数、高敏C反应蛋白（hs-CRP）、N末端脑钠肽前体（NT-proBNP）、空腹血糖、胆固醇水平、铁饱和度和叶酸浓度。使用电子顺磁共振光谱结合自旋探针CMH测定全血中超氧阴离子自由基的水平。采用国际体力活动问卷简表（IPAQ-SF）评估体力活动情况。对患者组进行血管功能评估（EndoPAT）和心肺运动试验。CHD成人患者与匹配对照组之间的超氧阴离子自由基水平无统计学显著差异。此外，氧化应激与CHD患者的炎症、内皮功能或心肺适能均无相关性；然而，观察到其与铁饱和度呈显著负相关。总体而言，CHD成人患者的全血超氧阴离子自由基水平并未升高，但铁水平在CHD氧化应激机制中似乎比在健康对照者中发挥着更重要的作用。需要开展更多研究以增进我们对CHD潜在病理生理学的理解。
