Center for Reproductive Medicine of New Mexico, Albuquerque, New Mexico 87106, USA.
Fertil Steril. 2010 Dec;94(7):2771.e1-3. doi: 10.1016/j.fertnstert.2010.04.071. Epub 2010 Jun 9.
To report a live birth after transfer of anonymously donated, twice-cryopreserved embryos that had been stored in liquid nitrogen for approximately 13.5 years.
Case report.
A private assisted reproduction center.
PATIENT(S): A 44-year-old recipient of donated cryopreserved embryos.
INTERVENTION(S): Anonymous donation of cryopreserved blastocysts for procreation.
MAIN OUTCOME MEASURE(S): Live birth after thawing and replacement of re-cryopreserved blastocysts.
RESULT(S): Fourteen pronuclear-stage embryos and four cleavage-stage embryos were cryopreserved during a primary IVF cycle. In two separate cycles, one cycle for the primary patient and a subsequent cycle for the first embryo donor recipient, the 18 embryos were thawed and grown to the blastocyst stage for transfer. Supernumerary blastocysts (n = 5) not replaced at either of these two thaw cycles were re-cryopreserved and subsequently donated to another embryo donor recipient. Five blastocysts survived the thaw and three were transferred, resulting in a live birth. The embryos were cryopreserved for a cumulative storage time of approximately 4,909 days (13.4 years).
CONCLUSION(S): The longevity (viability) of cryopreserved embryos maintained in liquid nitrogen remains to be determined; cryopreserved embryo donation for procreation should not be overlooked, regardless of the length of time that embryos remain in cryostorage.
报告一例匿名捐赠的、经过两次冷冻保存的胚胎解冻后移植并成功妊娠分娩的病例。这些胚胎在液氮中保存了约 13.5 年。
病例报告。
一家私立辅助生殖中心。
一名接受匿名捐赠冷冻胚胎的 44 岁受者。
匿名捐赠冷冻囊胚用于生育。
解冻和移植重新冷冻的囊胚后活产。
在首次体外受精（IVF）周期中，共冷冻保存了 14 枚原核期胚胎和 4 枚卵裂期胚胎。在两次单独的周期中，一次是为原发性患者，另一次是为首次胚胎供体受者，这 18 枚胚胎解冻并培养至囊胚阶段进行移植。在这两个解冻周期中，有 5 枚多余的囊胚未被移植，重新冷冻并随后捐赠给另一个胚胎供体受者。5 枚囊胚在解冻后存活，其中 3 枚被移植，最终成功妊娠分娩。这些胚胎的总冷冻保存时间约为 4909 天（13.4 年）。
冷冻保存胚胎在液氮中的长期（存活）能力仍有待确定；无论胚胎在冷冻储存中保留多长时间，冷冻胚胎捐赠用于生育都不应被忽视。
