Monteleone Centro de Reprodução Humana, São Paulo, SP, Brazil.
Departamento de Ginecologia. Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
JBRA Assist Reprod. 2021 Oct 4;25(4):644-646. doi: 10.5935/1518-0557.20210015.
Although frozen embryo transfer is a widely established route for assisted reproduction, successful frozen embryo transfer using embryos that have undergone long term cryopreservation remains relatively unexplored, and its efficacy remains a matter of some debate. This case report describes two successful frozen embryo transfer conceptions in the same patient, one after 3 months of cryopreservation and the second 10 years after cryopreservation. These embryos were cryopreserved using the slow freezing technique and were thawed using an unpaired technique (ultra-rapid warming) after 10 years of storage.
虽然冻融胚胎移植是辅助生殖的一种广泛应用的方法，但使用经过长期冷冻保存的胚胎进行成功的冻融胚胎移植仍然相对较少被探索，其疗效仍然存在一些争议。本病例报告描述了同一位患者的两次成功的冻融胚胎移植妊娠，一次是在冷冻保存 3 个月后，另一次是在冷冻保存 10 年后。这些胚胎使用慢速冷冻技术进行冷冻保存，在储存 10 年后使用非配对技术（超速融解）进行解冻。
