Laboratory of Plant Research, Department of Chemistry, Faculty of Science and Art, Gaziosmanpasa University, Taçlıçiftlik Campus, 60240 Tokat, Turkey.
Nat Prod Res. 2011 Sep;25(16):1512-23. doi: 10.1080/14786410903293191. Epub 2011 Jul 8.
Sideritis libanotica ssp. linearis was screened for the isolation of new natural antioxidant compounds. The antioxidant activity of flavones obtained from the methanol extract of the plant was evaluated in vitro using total antioxidant, reduction power and free radical scavenging activity. Results were compared with the positive controls of antioxidant standards (α-tocopherol and butylated hydroxytoluene). The results indicate that the flavones possess a higher antioxidant activity when compared to the other components in the plant. The lowest antioxidant activity was observed in fatty acids (FAs) and hydrocarbons. The FAs were methylated with MeOH and KOH and analysed by GC-MS. The structures of the isolated compounds were established based on spectroscopic evidence (NMR, GC-MS, HPLC, IR and UV). In this work, the isolated pure flavone (3'-O-methylhypolaetin 7-O-[6″'-O-acetyl-β-D-allopyranosyl-(1 → 2)]-6″-O-acetyl-β-D-glucopyranoside) was found to possess the highest antioxidant activity.
线性叶升麻亚种被筛选用于分离新的天然抗氧化化合物。采用总抗氧化能力、还原能力和自由基清除活性体外评价了从该植物甲醇提取物中获得的类黄酮的抗氧化活性。并将结果与抗氧化标准品（α-生育酚和丁基化羟基甲苯）的阳性对照进行了比较。结果表明，与植物中的其他成分相比，类黄酮具有更高的抗氧化活性。在脂肪酸 (FAs) 和碳氢化合物中观察到最低的抗氧化活性。FA 用 MeOH 和 KOH 甲基化并用 GC-MS 分析。根据光谱证据（NMR、GC-MS、HPLC、IR 和 UV）确定了分离化合物的结构。在这项工作中，分离得到的纯类黄酮（3'-O-甲基圣草酚 7-O-[6″'-O-乙酰基-β-D-吡喃半乳糖基-(1 → 2)]-6″-O-乙酰基-β-D-吡喃葡萄糖苷）被发现具有最高的抗氧化活性。
