Gonzalez Freddy L, Ranaivoson Hafaliana Christian, Andrianiaina Angelo, Andry Santino, Raharinosy Vololoniaina, Randriambolamanantsoa Tsiry Hasina, Lacoste Vincent, Dussart Philippe, Héraud Jean-Michel, Brook Cara E
Department of Ecology and Evolution, University of Chicago, IL, United States.
Department of Ecology and Evolutionary Biology, Yale University, CT, United States.
bioRxiv. 2024 Dec 24:2024.12.24.630179. doi: 10.1101/2024.12.24.630179.
Kobuviruses (family , genus ) are enteric viruses that infect a wide range of both human and animal hosts. Much of the evolutionary history of kobuviruses remains elusive, largely due to limited screening in wildlife. Bats have been implicated as major sources of virulent zoonoses, including coronaviruses, henipaviruses, and filoviruses, though much of the bat virome still remains uncharacterized. While most bat virus research has historically focused on immediately recognizable zoonotic clades (e.g. SARS-related coronaviruses), a handful of prior reports catalog kobuvirus infection in bats and posit the role of bats as potential progenitors of downstream kobuvirus evolution. As part of a multi-year study, we carried out metagenomic Next Generation Sequencing (mNGS) on fecal samples obtained from endemic, wild-caught Madagascar fruit bats to characterize potentially zoonotic viruses circulating within these populations. The wild bats of Madagascar represent diverse Asian and African phylogeographic histories, presenting a unique opportunity for viruses from disparate origins to mix, posing significant public health threats. Here, we report detection of kobuvirus RNA in Malagasy fruit bat () feces and undertake phylogenetic characterization of one full genome kobuvirus sequence, which nests within the Aichivirus A clade - a kobuvirus clade known to infect a wide range of hosts including humans, rodents, canids, felids, birds, and bats. Given the propensity of kobuviruses for recombination and cross-species infection, further characterization of this clade is critical to accurate evaluation of future zoonotic threats.
杯状病毒（科，属）是肠道病毒，可感染广泛的人类和动物宿主。杯状病毒的许多进化史仍然难以捉摸，这主要是由于对野生动物的筛查有限。蝙蝠被认为是包括冠状病毒、亨尼帕病毒和丝状病毒在内的烈性人畜共患病的主要来源，尽管许多蝙蝠病毒组仍未得到表征。虽然历史上大多数蝙蝠病毒研究都集中在易于识别的人畜共患病进化枝（如与SARS相关的冠状病毒）上，但此前有少数报告记录了蝙蝠中的杯状病毒感染，并认为蝙蝠是下游杯状病毒进化的潜在祖先。作为一项多年研究的一部分，我们对从马达加斯加当地野生捕获的果蝠粪便样本进行了宏基因组下一代测序（mNGS），以表征这些种群中传播的潜在人畜共患病病毒。马达加斯加的野生蝙蝠代表了不同的亚洲和非洲系统发育历史，为不同来源的病毒混合提供了独特的机会，对公共卫生构成了重大威胁。在这里，我们报告在马达加斯加果蝠（）粪便中检测到杯状病毒RNA，并对一个完整基因组的杯状病毒序列进行了系统发育表征，该序列嵌套在艾奇病毒A进化枝内——这是一个已知可感染包括人类、啮齿动物、犬科动物、猫科动物、鸟类和蝙蝠在内的广泛宿主的杯状病毒进化枝。鉴于杯状病毒易于重组和跨物种感染，对该进化枝进行进一步表征对于准确评估未来的人畜共患病威胁至关重要。
