Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 4400, Pakistan.
Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2469-76. doi: 10.1016/j.fct.2010.06.016. Epub 2010 Jun 13.
Sonchus asper (SA) is locally used in renal aliments. The present work investigated the antioxidant effects of S. asper methanolic extract (SAME) against CCl(4)-induced nephrotoxicity in Sprague-Dawley male rats. CCl(4) (3 ml/kg b.w., i.p.; 30% in olive oil) biweekly for 4 weeks induced lipid peroxidation, as reflected by significant increase of TBARS; diminished the renal antioxidant defenses, as revealed by a decrease of the level of GSH, CAT, SOD, GST, GSR, GSH-Px and QR while elevated the level of gamma-GT, H(2)O(2) and nitrite contents. CCl(4) caused histopathological injuries and significantly increased the renal AgNORs count and DNA damage. Telomerase activity in kidney was determined positive with CCl(4) treatment. Creatinine, urobilinogen and urea concentration was increased whereas creatinine clearance was decreased in serum and urine. Level of protein and albumin was increased in urine while reduced in serum. Serum level of nitrite was increased with CCl(4) treatment. Treatment of rats with SAME (100, 200mg/kg b.w.) effectively ameliorated the alterations induced with CCl(4) in lipid peroxidation, antioxidant defenses, biochemical markers, genotoxicity and renal lesions. The present data suggests that SAME protect the kidneys possibly by alleviating the oxidative stress induced with CCl(4) in rat.
沙蓬（SA）在当地被用于肾脏疾病。本研究调查了沙蓬甲醇提取物（SAME）对 CCl（4）诱导的雄性 SD 大鼠肾毒性的抗氧化作用。每周两次腹膜内注射 CCl（4）（3 ml/kg，30%橄榄油）共 4 周，导致脂质过氧化，反映为 TBARS 显著增加；降低了肾抗氧化防御能力，表现为 GSH、CAT、SOD、GST、GSR、GSH-Px 和 QR 水平降低，而γ-GT、H（2）O（2）和亚硝酸盐含量升高。CCl（4）引起组织病理学损伤，并显著增加肾 AgNORs 计数和 DNA 损伤。CCl（4）处理后肾端粒酶活性呈阳性。血清和尿液中肌酐、尿胆素原和尿素浓度升高，而肌酐清除率降低。尿液中蛋白质和白蛋白水平升高，而血清中降低。血清中亚硝酸盐水平升高与 CCl（4）处理有关。用 SAME（100、200mg/kg）治疗大鼠可有效改善 CCl（4）诱导的脂质过氧化、抗氧化防御、生化标志物、遗传毒性和肾脏病变的改变。本研究数据表明，SAME 可能通过减轻 CCl（4）诱导的大鼠氧化应激来保护肾脏。
