El-Beltagi Hossam S, Rageb Marwa, El-Saber Mahmoud M, El-Masry Ragab A, Ramadan Khaled M A, Kandeel Mahmoud, Alhajri Ahlam Saleh, Osman Ali
Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, 31982, Saudi Arabia.
Biochemistry Department, Cairo University, Giza, 12613, Egypt.
Heliyon. 2024 May 3;10(9):e30627. doi: 10.1016/j.heliyon.2024.e30627. eCollection 2024 May 15.
Hepatotoxin carbon tetrachloride (CCl) causes liver injury. This research aims to create ZnO-NPs using green synthesis from (MO) leaves aqueous extract, and chemically prepared and confirming the synthesis by specialized equipment analysis. The sizes formed of ZnO-NPs were 80 and 55 nm for chemical and green methods, respectively. In addition, to study their ability to protect Wistar Albino male rats against oxidative stress exposed to carbon tetrachloride. MO leaf aqueous extract, green synthesized ZnO-NPs, and ZnO-NPs prepared chemically at 100 and 200 mg/kg BW per day were investigated for their hepatoprotective effects on liver enzyme biomarkers, renal biomarkers, antioxidant enzymes, lipid peroxidation, hematological parameters, and histopathological changes. Compared to the control group, all liver and kidney indicators were considerably elevated after the CCl injection. However, the activity of antioxidant enzymes in the liver was significantly reduced after the CCl injection. These outcomes indicate that MO leaf aqueous extract, greenly synthesized ZnO-NPs, and ZnO-NPs chemically prepared can restore normal liver and kidney function and activity, as well as hematological and antioxidant enzymes. The highest impact on enhancing the hepatoprotective effect was recorded for rats that received green synthesized ZnO-NPs. The increased drug delivery mechanism of green synthesized ZnO-NPs resulted in a higher protective effect than that of MO leaf aqueous extract.
肝毒素四氯化碳（CCl）会导致肝损伤。本研究旨在利用（MO）叶水提取物通过绿色合成法制备氧化锌纳米颗粒（ZnO-NPs），并通过专业设备分析进行化学制备和合成确认。化学法和绿色法制备的ZnO-NPs的尺寸分别为80纳米和55纳米。此外，研究它们保护Wistar白化雄性大鼠免受四氯化碳所致氧化应激的能力。对MO叶水提取物、绿色合成的ZnO-NPs以及每天按100和200毫克/千克体重化学制备的ZnO-NPs对肝酶生物标志物、肾生物标志物、抗氧化酶、脂质过氧化、血液学参数和组织病理学变化的肝保护作用进行了研究。与对照组相比，注射CCl后所有肝脏和肾脏指标均显著升高。然而，注射CCl后肝脏中抗氧化酶的活性显著降低。这些结果表明，MO叶水提取物、绿色合成的ZnO-NPs以及化学制备的ZnO-NPs可以恢复肝脏和肾脏的正常功能及活性，以及血液学和抗氧化酶的正常水平。接受绿色合成的ZnO-NPs的大鼠在增强肝保护作用方面的影响最大。绿色合成的ZnO-NPs增加的药物递送机制导致其保护作用比MO叶水提取物更高。
