Bornstein N, Marmet D, Dumaine M H, Surgot M, Fleurette J
Centre National de Référence des Légionelloses, Faculté de Médecine Alexis Carrel, Lyon, France.
J Clin Microbiol. 1991 May;29(5):953-6. doi: 10.1128/jcm.29.5.953-956.1991.
To determine whether all Legionella species show common flagellum antigen properties, we developed a reagent using latex beads sensitized with flagellin-specific immunoglobulins that could be used in a simple and rapid agglutination reaction to identify Legionella colonies. A total of 278 strains (68 Legionella reference strains and 210 patient and environmental isolates) were tested. The results were compared with those obtained by a direct immunofluorescence assay using an antiflagellum serum and by morphological observations by electron microscopy. The immunological methods based on the use of a flagellum-specific serum have confirmed the presence of a common flagellum antigen for all Legionella species described to date. Flagella were detected for all the legionellae studied except four species: L. oakridgensis, confirmed as a nonflagellate species; L. brunensis; L. cincinnatiensis; and L. longbeachae serogroup 1. However, we noted a remarkable variability in flagellum expression, of greater or lesser degree, according to the species and their origin. A combination of all three methods of flagellum detection revealed that 86.3% of Legionella strains studied were flagellate. The latex test identified 89.6% of these strains, 97.5% of L. pneumophila, and 100% of L. pneumophila serogroup 1.
为了确定所有军团菌属是否具有共同的鞭毛抗原特性，我们研发了一种试剂，该试剂使用用鞭毛蛋白特异性免疫球蛋白致敏的乳胶珠，可用于简单快速的凝集反应以鉴定军团菌菌落。共检测了278株菌株（68株军团菌参考菌株以及210株患者和环境分离株）。将结果与使用抗鞭毛血清的直接免疫荧光检测以及电子显微镜形态学观察所获得的结果进行比较。基于使用鞭毛特异性血清的免疫学方法已证实，迄今所描述的所有军团菌属均存在共同的鞭毛抗原。除了四种菌株外，在所研究的所有军团菌中均检测到了鞭毛：橡树岭军团菌，已确认为无鞭毛菌株；布鲁内斯军团菌；辛辛那提军团菌；以及长滩军团菌血清群1。然而，我们注意到，根据菌株种类及其来源，鞭毛表达存在显著程度不一的变异性。三种鞭毛检测方法相结合显示，所研究的军团菌菌株中有86.3%具有鞭毛。乳胶试验鉴定出了这些菌株中的89.6%、嗜肺军团菌的97.5%以及嗜肺军团菌血清群1的100%。
