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Enzyme immunoassay for detection of antibodies against isolated flagella of Legionella pneumophila.

作者信息

Neumeister B, Susa M, Nowak B, Straube E, Ruckdeschel G, Hacker J, Marre R

机构信息

Abteilung Medizinische Mikrobiologie und Hygiene, Universität Ulm, Germany.

出版信息

Eur J Clin Microbiol Infect Dis. 1995 Sep;14(9):764-7. doi: 10.1007/BF01690990.

DOI:10.1007/BF01690990
PMID:8536723
Abstract

Flagella of Legionella pneumophila serogroup 1, strain Philadelphia (ATCC 33152), were isolated and used as antigen to detect antibodies by enzyme immunoassay (EIA) in sera of patients with significant titers against Legionella as determined by the indirect immunofluorescence assay (IFA). Healthy blood donors served as a control group. Whereas the sera of blood donors were negative in both assays, sera of patients showed two patterns of reactivity. Sera with elevated titers against Legionella pneumophila serogroup 1 or Legionella gormanii in IFA usually demonstrated high titers in EIA. However, high IFA titers against Legionella bozemanii were associated with low titers in EIA. The data show that flagella protein of Legionella pneumophila serogroup 1 strain Philadelphia is of limited value for the detection of antibodies against Legionella other than Legionella pneumophila serogroup 1, although in vitro studies revealed genus-specific epitopes. This might be due to unpredictable variabilities in the expression of flagella in vivo.

摘要

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Antigens of Borrelia burgdorferi recognized during Lyme disease. Appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness.莱姆病期间识别出的伯氏疏螺旋体抗原。疾病后期出现新的免疫球蛋白M反应以及免疫球蛋白G反应的扩大。
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